Zic1 negatively regulates brown adipogenesis in C3H10T1/2 cells
Zic1 negatively regulates brown adipogenesis in C3H10T1/2 cells
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Zic1 负向调节 C3H10T1/2 细胞中的棕色脂肪生成
DOI：
10.1007/s11434-015-0797-9
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发表时间：
2015-05
期刊：
影响因子：
18.9
通讯作者：
Jin Wanzhu
中科院分区：
文献类型：
--
作者：
Zhang Hanlin;Huang Yuanyuan;Lee Hyuek Jong;Jin Wanzhu
小脑锌指蛋白1（Zic1）已知在发育阶段调节神经发生和肌发生，并被广泛用作棕色脂肪细胞特异性标志物之一。在这项研究中，我们检测了Zic1对棕色脂肪形成的影响。过表达……
Zinc finger in the cerebellum 1 (Zic1) is known to regulate neurogenesis and myogenesis in the developmental stage and widely used as one of the brown adipocyte-specific markers. In this study, we examined the effect of Zic1 on brown adipogenesis. Overexpression of Zic1 attenuated the lipid accumulation and the expressions of PPARγ2 and C/EBPα in C3H10T1/2 mesenchymal stem cells. The mRNA levels of BAT-specific thermogenic genes (PRDM16, PGC-1α and UCP1) and fatty acid oxidation regulatory genes (PPARα, CPT1α, CPT1β and COX7α1) were suppressed in Zic1-overexpressed cells. Moreover, overexpression of Zic1 reduced the mitochondrial oxidative phosphorylation (OXPHOS) regulatory proteins including ATP5α, UQCRC2, SDHB and NDUFB5. These results indicate a potential role of Zic1 in the regulation of brown adipogenesis via inhibiting adipogenesis, fatty acid oxidation and mitochondrial OXPHOS.