Optical-resolution photoacoustic microscopy for monitoring vascular normalization during anti-angiogenic therapy
Optical-resolution photoacoustic microscopy for monitoring vascular normalization during anti-angiogenic therapy
复制标题
光学分辨率光声显微镜用于监测抗血管生成治疗期间血管正常化。
DOI：
10.1016/j.pacs.2019.100143
复制
发表时间：
2019-09-01
期刊：
影响因子：
7.9
通讯作者：
Zheng, Rongqin
中科院分区：
文献类型：
--
作者：
Zhou, Hui-Chao;Chen, Ningbo;Zheng, Rongqin
监测肿瘤血管分布的变化对于评估抗血管生成治疗具有重要意义。然而，由于缺乏适当的成像技术，监测血管是相当具有挑战性的。在这里，我们描述了一种非侵入性成像技术，使用光学分辨率光声显微镜（OR-PAM）跟踪血管的变化，在前列腺癌治疗与抗血管生成剂，DC 101，小鼠耳异种移植模型。初始治疗后约1-3天，OR-PAM成像检测到肿瘤血管变化，例如血管曲折度降低、血管直径减小和瘤内血管分布均匀。这些观察结果表明血管正常化，这在病理学上被证实为增加的周细胞覆盖率、功能灌注和血管的药物递送。在4次DC 101干预后，OR-PAM成像最终显示肿瘤内血管消退。因此，血管的OR-PAM成像提供了一种有前途的方法来研究体内抗血管生成药物的作用机制，并在监测和指导抗血管生成治疗方面具有潜力。
Monitoring the changes in tumor vascularity is important for anti-angiogenic therapy assessment with therapeutic implications. However, monitoring vascularity is quite challenging due to the lack of appropriate imaging techniques. Here, we describe a non-invasive imaging technique using optical-resolution photoacoustic microscopy (OR-PAM) to track vascular changes in prostate cancer treated with an anti-angiogenic agent, DC101, on a mouse ear xenograft model. Approximately 1-3 days after the initial therapy, OR-PAM imaging detected tumor vascular changes such as reduced vessel tortuosity, decreased vessel diameter and homogenized intratumoral vessel distribution. These observations indicated vessel normalization, which was pathologically validated as increased fractional pericyte coverage, functional perfusion and drug delivery of the vessels. After four DC101 interventions, OR-PAM imaging eventually revealed intratumoral vessel regression. Therefore, OR-PAM imaging of the vasculature offers a promising method to study anti-angiogenic drug mechanisms of action in vivo and holds potential in monitoring and guiding anti-angiogenic therapy.