MicroRNA transcriptome analysis in chicken kidneys in response to differing virulent infectious bronchitis virus infections
MicroRNA transcriptome analysis in chicken kidneys in response to differing virulent infectious bronchitis virus infections
复制标题
鸡肾响应不同烈性传染性支气管炎病毒感染的 MicroRNA 转录组分析
DOI：
10.1007/s00705-017-3502-2
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发表时间：
2017-11-01
影响因子：
2.7
通讯作者：
Wang, Hongning
中科院分区：
文献类型：
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作者：
Yang, Xin;Gao, Wenqian;Wang, Hongning
传染性支气管炎病毒（IBV）可在家禽中引起高度传染性和急性呼吸道疾病。MicroRNAs （miRNAs）已成为一类重要的基因表达调控因子，并参与病毒防御和免疫过程的调控。为了了解鸡对IBV感染的miRNA调控，我们进行了高通量测序，比较了感染SCK2、SCDY2和LDT3-A的鸡肾脏的小RNA文库。通过将这些数据与健康鸡进行比较，共鉴定出58个差异表达（DE） mirna。DE miRNA进一步分为五种miRNA表达模式（与对照组相比上调或下调）。通过基因本体（Gene Ontology， GO）富集预测，DE mirna主要与代谢过程、催化活性、基因表达、结合活性和免疫反应相关。选择7个高表达的mirna （gga-miR-30d、gga-miR-1454、gga-miR-7b、gga-miR-215-5p、gga-miR-1a-3p、gga-miR-3538和gga-miR-2954）进行miRNA-mRNA联合分析。此外，mirna与相应的靶基因mrna呈负相关。这7种mirna被认为在IBV-宿主相互作用和IBV菌株的不同毒力中起重要作用。这是首次证明不同毒力ibv感染引起鸡肾脏中miRNAs的不同表达；这种表达似乎也与IBV的毒力有关。这些结果对于研究mirna介导的不同毒力IBV感染的免疫应答以及鸡宿主与IBV的相互作用具有重要意义。
Infectious bronchitis virus (IBV) can cause a highly contagious and acute respiratory disease in poultry. MicroRNAs (miRNAs) have emerged as a class of crucial regulators for gene expression and are involved in the regulation of virus defence and immunological processes. To understand miRNA regulation in chickens in response to IBV infection, high-throughput sequencing was performed to compare the small RNA libraries from the kidneys of chicken infected with SCK2, SCDY2 and LDT3-A. By comparing these data to healthy chickens, a total of 58 differentially expressed (DE) miRNAs were identified. The DE miRNAs were further classified into five miRNA expression patterns (up or down regulation compared to control). Using Gene Ontology (GO) enrichment prediction, the DE miRNAs were shown to be mostly associated with metabolic processes, catalytic activities, gene expression, binding activities and immune responses. Seven highly expressed miRNAs (gga-miR-30d, gga-miR-1454, gga-miR-7b, gga-miR-215-5p, gga-miR-1a-3p, gga-miR-3538 and gga-miR-2954) were selected for miRNA-mRNA conjoint analysis. Furthermore, the miRNAs inversely correlated with the corresponding target gene mRNAs. These seven miRNAs were considered to play an important role in IBV-host interactions and the differing virulence of IBV strains. This is the first demonstration that infection with different virulent IBVs elicits different expression of miRNAs in chicken kidneys; this expression also seems to be associated with the virulence of IBV. These results are significant for the study of immune responses to infection with different virulent IBVs mediated by miRNAs as well as the interaction between the chicken host and IBV.