miR-429-3p/LPIN1 Axis Promotes Chicken Abdominal Fat Deposition via PPARγ Pathway.
miR-429-3p/LPIN1 Axis Promotes Chicken Abdominal Fat Deposition via PPARγ Pathway.
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miR-429-3p/LPIN1 轴通过 PPARγ 途径促进鸡腹部脂肪沉积。
DOI：
10.3389/fcell.2020.595637
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发表时间：
2020
影响因子：
5.5
通讯作者：
Luo Q
中科院分区：
文献类型：
--
作者：
Chao X;Guo L;Wang Q;Huang W;Liu M;Luan K;Jiang J;Lin S;Nie Q;Luo W;Zhang X;Luo Q
为探讨肉鸡腹脂沉积的调控机制，选用100日龄三黄鸡12只，按腹脂比大小分为高脂组和低脂组。从12例腹部脂肪组织中提取总RNA，进行miRNA和mRNA测序。MiRNA和mRNA测序结果表明，miR-429-3p在高脂鸡中高表达，而LPIN1在高脂鸡中表达下调。进一步，我们确定miR-429-3p促进前脂肪细胞的增殖和分化，而LPIN1则起相反的作用。值得注意的是，我们发现miR429-3p/LPIN1轴促进了PPARγ途径的激活，这与脂肪形成的进展密切相关。综上所述，我们的研究结果为新的miR-429-3p/LPIN1轴参与脂肪生成的调控提供了证据，这可能对肉鸡腹脂性状的育种具有指导作用。
To explore the regulatory mechanism of abdominal fat deposition in broilers, 100-day-old Sanhuang chickens (n = 12) were divided into high-fat and low-fat groups, according to the abdominal fat ratio size. Total RNA isolated from the 12 abdominal fat tissues was used for miRNA and mRNA sequencing. Results of miRNA and mRNA sequencing revealed that miR-429-3p was highly expressed in high-fat chicken whereas LPIN1 expression was downregulated. Further, we determined that miR-429-3p promoted preadipocyte proliferation and differentiation, whereas LPIN1 exerted an opposite effect. Notably, we found that the miR-429-3p/LPIN1 axis facilitated PPARγ pathway activation, which is closely associated with the progression of adipogenesis. In conclusion, our results provide evidence that a novel miR-429-3p/LPIN1 axis is involved in the regulation of adipogenesis, which may have a guiding role in the improvement of breeding for abdominal fat traits in broiler chickens.