Remodeling with neointima formation in the mouse carotid artery after cessation of blood flow
Remodeling with neointima formation in the mouse carotid artery after cessation of blood flow
复制标题
DOI：
10.1161/01.atv.17.10.2238
复制
发表时间：
1997-10-01
影响因子：
8.7
通讯作者：
Lindner, V
中科院分区：
文献类型：
--
作者：
Kumar, A;Lindner, V
在小鼠物种中，基因靶向的能力为确定特定分子在血管生物学中的作用提供了有力的工具。使用剥脱损伤程序，我们最近报道了可以在远交种小鼠的颈动脉中诱导内膜病变。实现完全剥脱的技术挑战和相对较小的病变发展促使我们设计了近交系FVB小鼠颈动脉新内膜形成和血管重塑的模型。在颈动脉分叉处血管的完全结扎诱导了内侧平滑肌细胞的快速增殖，在内皮层的存在下导致广泛的新内膜形成。除了血管最末端与结扎处相邻外，未观察到血栓形成。结扎后4周，由于血管直径减小和新生内膜形成，管腔面积减少了约80%。超微结构分析提供了新生内膜细胞死亡和重构介质的证据。目前的模型可能有助于识别那些对新内膜形成和血管重塑重要的基因。
The ability of gene targeting in the mouse species presents a powerful tool to determine the role of specific molecules in vascular biology. Using a denuding-injury procedure, we recently reported that intimal lesions can be induced in the carotid artery of outbred mice. The technical challenge associated with achieving complete denudation and the relatively small size of the developing lesions prompted us to design the present model of neointima formation and vascular remodeling in the carotid artery of the inbred FVB mouse strain. Complete ligation of the vessel near the carotid bifurcation induced rapid proliferation of medial smooth muscle cells, leading to extensive neointima formation in the presence of an endothelial lining. Thrombus formation was not observed except in the most distal part of the vessel adjacent to the ligature. At 4 weeks after ligation, luminal area was reduced by approximate to 80% through a combination of decreased vessel diameter and neointima formation. Ultrastructural analysis provided evidence for cell death in the developing neointima as well as the remodeling media. The present model might be useful in identifying those genes important for neointima formation and vascular remodeling.