Purification and identification of a BMP-like factor from bovine serum
Purification and identification of a BMP-like factor from bovine serum
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DOI：
10.1016/j.bbrc.2006.05.045
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发表时间：
2006-07-07
影响因子：
3.1
通讯作者：
Katagiri, Takenobu
中科院分区：
文献类型：
--
作者：
Kodaira, Kunihiko;Imada, Mana;Katagiri, Takenobu
肌原性分化在体外被胎牛血清（FBS）中存在的未知因子抑制。我们发现，骨形态发生蛋白（BMP）的特异性抑制剂刺激成肌分化，即使在20%FBS的存在下，这反过来又激活了特定的BMP信号。此外，这些特异性BMP抑制剂阻断FBS诱导的成骨细胞成熟，表明血清中BMP样因子调节肌原性和成骨细胞分化。确定的因素有一个表观分子量（Mw）超过100 kDa的Superdex 200柱分子筛HPLC，但在非还原条件下的SDS-PAGE上的表观分子量为3 - 3 kDa。通过液相色谱-串联质谱法分析从FBS（5L）纯化的制剂，揭示了在成熟的人和鼠BMP-4之间保守的氨基酸序列的存在。这是第一项研究表明BMP-4作为一个大的复合物存在于FBS中。(c)2006爱思唯尔公司All rights reserved.
Myogenic differentiation is suppressed in vitro by unknown factors present in fetal bovine serum (FBS). We found that specific inhibitors of bone morphogenetic proteins (BMPs) stimulated myogenic differentiation even in the presence of 20% FBS, which in turn activated specific BMP signaling. Moreover, these specific BMP inhibitors blocked maturation of osteoblastic cells induced by FBS, indicating that BMP-like factor(s) in serum regulate both myogenic and osteoblastic differentiation. The factor identified had an apparent molecular weight (Mw) of over 100 kDa on a Superdex 200 column for molecular sieving HPLC, but an apparent Mw of 3 3 kDa on SDS-PAGE under non-reducing conditions. Analysis of a purified preparation from FBS (5 L) by liquid chromatography-tandem mass spectrometry revealed the presence of an amino acid sequence conserved between mature human and murine BMP-4. This is the first study to show that BMP-4 is present in FBS as a large complex. (c) 2006 Elsevier Inc. All rights reserved.