Persistent replication of the GBV-C subgenomic replicons in Huh7 cells
Persistent replication of the GBV-C subgenomic replicons in Huh7 cells
复制标题
GBV-C 亚基因组复制子在 Huh7 细胞中持续复制。
DOI：
10.1016/j.jviromet.2008.12.020
复制
发表时间：
2009-05-01
影响因子：
3.1
通讯作者：
Qi, Zhong-Tian
中科院分区：
文献类型：
--
作者：
Cao, Ming-Mei;Ren, Hao;Qi, Zhong-Tian
GB病毒C型（GBV-C）的体外复制研究受到限制，因为GBV-C在细胞培养中生长不良。为了解决GBV-C的感染，从GBV-C全长基因组cDNA中获得了两个GBV-C亚基因组复制子（GBCrepEGFP和GBCrepTNF）。研究了人肝癌细胞株Huh7的病毒复制、蛋白表达和病毒样颗粒的产生。结果表明，构建的GBCrepEGFP和GBCrepTNF复制子可在细胞培养中自主复制，表达时间分别至少为2个月和1个月。复制子RNA可以在表达GBV-C结构蛋白的HuhEH细胞中组装含RNA的结构。提示建立了支持GBV-C复制的细胞系。该复制子系统可用于更好地了解GBV-C的生物学特性。(C) 2008 Elsevier B.V.版权所有
Studies of GB virus type C (GBV-C) replication in vitro have been limited because of poor growth of GBV-C in cell culture. In order to address the infection of GBV-C, two GBV-C subgenomic replicons (GBCrepEGFP and GBCrepTNF) were developed from a GBV-C full-length genomic cDNA. The viral replication, protein expression and the production of virus-like particles were evaluated in human hepatoma cell line Huh7. The results showed that the established GBCrepEGFP and GBCrepTNF replicons could be replicated autonomously and expressed in cell culture for at least 2 months and I month respectively. The replicon RNA could assemble RNA-containing structures in the HuhEH cells expressing GBV-C structural proteins. It suggests that a cell line Supporting the replication of GBV-C was established. This replicon system might be used to understand better the biology of GBV-C. (C) 2008 Elsevier B.V. All rights reserved.