Cortical spreading depression affects Fos expression in the hypothalamic paraventricular nucleus and the cerebral cortex of both hemispheres
Cortical spreading depression affects Fos expression in the hypothalamic paraventricular nucleus and the cerebral cortex of both hemispheres
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DOI：
10.1016/s0168-0102(02)00207-9
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发表时间：
2003-02-01
影响因子：
2.9
通讯作者：
Nakamura, S
中科院分区：
文献类型：
--
作者：
Chowdhury, GMI;Liu, Y;Nakamura, S
本实验的目的是澄清大脑部位的活动仅受皮质扩散抑制（CoSD）的影响。为此，使用立即早期基因的产物 Fos 蛋白作为神经元激活的标记。由于 Fos 可以通过应激刺激等多种操作诱导，因此我们通过排除麻醉和手术操作等其他因素的影响来验证 CoSD 诱导的 Fos 表达。通过将 KCl 溶液直接施加到大脑皮层上的硬脑膜来诱导 CoSD，并使用针对 Fos 蛋白的抗体通过免疫组织化学评估大脑中的 Fos 表达。我们发现，在 CoSD 期间，Fos 表达在下丘脑室旁核 (PVN) 的大细胞区域以及同侧皮质中特别增加，而在 PVN 的小细胞区域和 CoSD 部位对侧的整个皮质中均观察到 Fos 表达减少。与 Fos 表达减少一致，CoSD 期间对侧皮质中约 40% 的神经元显示电活动受到抑制。这些结果表明，除了同侧皮质外，CoSD 仅影响 PVN 和对侧皮质中的 Fos 表达。 (C) 2002 Elsevier Science Ireland Ltd 和日本神经科学学会。版权所有。
The present experiments were performed to clarify the brain sites whose activity is affected exclusively by cortical spreading depression (CoSD). For this purpose, Fos protein, a product of an immediate early gene, was used as a marker of neuronal activation. Because Fos can be induced by many manipulations such as stress stimuli, we verified CoSD-induced Fos expression by excluding the influence of other factors such as anaesthesia and surgical manipulation. CoSD was induced by applying a KCl solution directly to the dura mater over the cerebral cortex, and Fos expression in the brain was assessed by immunohistochemistry using antibodies against Fos protein. We found that during CoSD, Fos expression was increased specifically in the magnocellular region of the hypothalamic paraventricular nucleus (PVN), as well as in the ipsilateral cortex, whereas reduced Fos expression was observed in both the parvocellular region of the PVN and the whole cortex contralateral to the CoSD site. Consistent with the reduced Fos expression, approximately 40% of neurons in the contralateral cortex revealed a suppression of electrical activity during CoSD. These results suggest that in addition to the ipsilateral cortex, CoSD affects Fos expression exclusively in the PVN and the contralateral cortex. (C) 2002 Elsevier Science Ireland Ltd and the Japan Neuroscience Society. All rights reserved.