Human monoclonal antibody against Forssman antigen.
Human monoclonal antibody against Forssman antigen.
复制标题
抗福斯曼抗原的人单克隆抗体。
作者：
R. Nowinski;C. Berglund;J. Lane;M. Lostrom;I. Bernstein;W. Young;S. Hakomori;L. Hill;M. Cooney
人淋巴细胞和小鼠骨髓瘤细胞之间形成的杂交细胞在培养中产生人免疫球蛋白。稳定的抗体产生细胞系可以在多个循环的低密度传代、克隆和用于免疫球蛋白产生的连续选择后分离。描述了人和小鼠细胞杂交的起源和特征。这种杂交产生高浓度（8.3微克/毫升）的人免疫球蛋白M与福斯曼糖脂的末端二糖反应。这些发现指出了人-小鼠杂交细胞作为用于治疗和诊断目的的人单克隆抗体的来源的潜在用途。
Hybrid cells formed between human lymphocytes and mouse myeloma cells produce human immunoglobulin in culture. Stable antibody-producing cell lines can be isolated after multiple cycles of low-density passage, cloning, and continued selection for immunoglobulin production. The origin and characteristics of a hybrid of human and mouse cells is described. This hybrid produces high concentrations (8.3 micrograms per milliliter) of human immunoglobulin M reactive with the terminal disaccharide of the Forssman glycolipid. These findings point to the potential use of human-mouse hybrid cells as a source of human monoclonal antibodies for therapeutic and diagnostic purposes.