TNF-α gene expression in the liver of the IFN-γ transgenic mouse with chronic active hepatitis
TNF-α gene expression in the liver of the IFN-γ transgenic mouse with chronic active hepatitis
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慢性活动性肝炎IFN-γ转基因小鼠肝脏中TNF-α基因的表达
DOI：
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发表时间：
1996
期刊：
影响因子：
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通讯作者：
O. Hino
中科院分区：
文献类型：
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作者：
T. Okamoto;M. Furuya;T. Yamakawa;K. Yamamura;O. Hino
用聚合酶链式反应检测特异性表达干扰素-γ基因(干扰素-γ)转基因小鼠肝脏细胞因子基因的表达。转基因小鼠肝脏中同时表达干扰素-γ基因和肿瘤坏死因子-α基因。T细胞成熟标志物CD4和CD8的信使RNA在正常和转基因小鼠肝脏中均有表达，表明存在常驻T细胞。在转基因小鼠中，CD4和CD8的表达水平略有上升，表明CD4+和/或CD8+T细胞的数量增加，但缺乏激活标记IL-2mRNA表明处于非激活状态。转化生长因子-β1mRNA在正常小鼠和转基因小鼠肝脏中均有表达，而转基因小鼠肝脏中的表达没有增加，因此不参与该转基因小鼠慢性活动性肝炎的发生。由于肿瘤坏死因子-α在几种动物肝损伤模型中是肝损伤的终末介质，其高表达可能是从导入的基因转录而来的干扰素-γ的结果，可能参与了该转基因小鼠慢性活动性肝炎的发展。
Cytokine gene expression in the liver of transgenic mouse which specifically expresses interferon-γ gene (IFN-γ) was measured using polymerase chain reaction analysis. IFN-γ mRNA was found to be expressed in the transgenic mouse liver along with TNF-α mRNA. Messenger RNAs of CD4 and CD8, maturation markers of T cells, were expressed in both normal and transgenic mouse livers, indicating the presence of resident T cells. The level of CD4 and CD8 expression was slightly elevated in the transgenic mouse case, indicating an increased number of CD4+and/or CD8+T cells, but the lack of the activation marker IL-2 mRNA suggested a nonactivated state. TGF-β1 mRNA was expressed in both normal and transgenic mouse livers without any increase in the latter, and therefore does not appear to be involved in the development of chronic active hepatitis in this transgenic mouse. Since TNF-α is a terminal mediator of liver injury in several animal liver injury models, its high expression, presumably a result of the IFN-γ which is transcribed from the introduced gene, might be involved in the development of chronic active hepatitis in this transgenic mouse.