Screening of antigen-specific antibody-secreting cells.
Screening of antigen-specific antibody-secreting cells.
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DOI：
10.1007/978-1-61779-567-1_11
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发表时间：
2012
影响因子：
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通讯作者：
H. Kishi;A. Jin;Tatsuhiko Ozawa;K. Tajiri;Tsutomu Obata;A. Muraguchi
中科院分区：
文献类型：
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作者：
H. Kishi;A. Jin;Tatsuhiko Ozawa;K. Tajiri;Tsutomu Obata;A. Muraguchi
抗原特异性抗体产生细胞的筛选是获得抗原特异性单抗的关键步骤。在小鼠系统中，B淋巴细胞和骨髓瘤细胞之间的杂交瘤被用来筛选和产生抗原特异性的单抗。在人类系统中，没有良好的杂交瘤产生系统。取而代之的是，用EB病毒转化B淋巴细胞来获得抗体分泌细胞系。此外，基于分子生物学的噬菌体展示系统最近被用于获得抗原特异性的人源单抗。在这里，我们描述了使用微孔阵列芯片在单细胞水平上筛选抗原特异性抗体分泌细胞的新方法。该系统可用于从任何动物物种中筛选抗原特异性抗体分泌细胞。
Screening of antigen-specific antibody-producing cells is a key step for obtaining antigen-specific monoclonal antibodies. In murine system, hybridoma between B-lymphocytes and myeloma cells is used to screen and produce antigen-specific monoclonal antibodies. In human system, good hybridoma-producing system is not available. Instead, transformation of B-lymphocytes with Epstein–Barr viruses is used to obtain antibody-secreting cell lines. Furthermore, phage-display system using molecular biology is recently used to obtain antigen-specific human monoclonal antibodies. Here, we describe the new method for screening antigen-specific antibody-secreting cells at single-cell levels using microwell-array chips. The system can be applied to screen antigen-specific antibody-secreting cells from any animal species.