INABILITY OF MOUSE BLASTOMERE NUCLEI TRANSFERRED TO ENUCLEATED ZYGOTES TO SUPPORT DEVELOPMENT INVITRO
INABILITY OF MOUSE BLASTOMERE NUCLEI TRANSFERRED TO ENUCLEATED ZYGOTES TO SUPPORT DEVELOPMENT INVITRO
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DOI：
10.1126/science.6542249
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发表时间：
1984-01-01
期刊：
影响因子：
56.9
通讯作者：
SOLTER, D
中科院分区：
文献类型：
--
作者：
MCGRATH, J;SOLTER, D
90%以上的去核小鼠1-细胞胚胎接受其他1-细胞胚胎的原核后，在体外成功发育到囊胚期。将来自连续的植入前卵裂阶段的细胞核引入去核的1-细胞胚胎中，并对胚胎进行体外发育测试。虽然2细胞核支持发育到桑椹胚或囊胚期，4细胞，8细胞和内细胞团细胞核没有。来自这些阶段的细胞核不能支持发育反映了转移的细胞核的全能性的快速丧失，并且不是膜或细胞质同时转移的结果。
More than 90% of enucleated 1-cell mouse embryos receiving pronuclei from other 1-cell embryos successfully develop to the blastocyst stage in vitro. Nuclei from successive preimplantation cleavage stages were introduced into enucleated 1-cell embryos and the embryos were tested for development in vitro. Although 2-cell nuclei supported development to the morula or blastocyst stage, 4-cell, 8-cell and inner cell mass cell nuclei did not. The inability of cell nuclei from these stages to support development reflects rapid loss of totipotency of the transferred nucleus and is not the result of simultaneous transfer of membrane or cytoplasm.