COGENERATION OF RETROGRADELY LABELED CORTICOCORTICAL PROJECTION AND GABA-IMMUNOREACTIVE LOCAL CIRCUIT NEURONS IN CEREBRAL-CORTEX
COGENERATION OF RETROGRADELY LABELED CORTICOCORTICAL PROJECTION AND GABA-IMMUNOREACTIVE LOCAL CIRCUIT NEURONS IN CEREBRAL-CORTEX
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DOI：
10.1016/0165-3806(85)90040-9
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发表时间：
1985-12-01
期刊：
影响因子：
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通讯作者：
MILLER, MW
中科院分区：
文献类型：
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作者：
MILLER, MW
测定大鼠视觉皮层皮质-皮质投射神经元和局部环路神经元的起源时间。使用逆行标记与凝集素结合的辣根过氧化物酶和氚化胸苷放射自显影相结合的技术来评估投射神经元的出生日期。通过结合GABA免疫细胞化学和[3H]胸苷放射自显影来确定一些皮质局部回路神经元的出生日期。双标记神经元（核周带有逆行或免疫反应标记且细胞核上有放射自显影银粒的神经元）在妊娠第三周出生。妊娠第 14、15、17、19 或 20 天出生的投射神经元和局部环路神经元分别主要位于 VIb、VIa、V、III 或 II 层。因此，两个神经元群都是通过并行且并发的从内到外模式生成的。
The times of origin of cortico-cortical projection neurons and local circuit neurons in rat visual cortex were determined. The birthdates of the projection neurons were assessed using a technique that combined retrograde labeling with lectin-bound horseradish peroxidase and tritiated thymidine autoradiography. The birthdates of some cortical local circuit neurons were determined by combining GABA immunocytochemistry with [3H]thymidine autoradiography. Double-labeled neurons (those with retrograde or immunoreactive label in their perikarya and autoradiographic silver grains over their nuclei) were born during the third week of gestation. Projection and local circuit neurons born on gestational days 14, 15, 17, 19 or 20 were located primarily in layer VIb, VIa, V, III or II, respectively. Thus, both populations of neurons are generated by parallel and concurrent inside-to-outside patterns.