A cellular platform for the development of synthetic living machines
A cellular platform for the development of synthetic living machines
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DOI：
10.1126/scirobotics.abf1571
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发表时间：
2021-03-17
期刊：
影响因子：
25
通讯作者：
Levin, Michael
中科院分区：
文献类型：
--
作者：
Blackiston, Douglas;Lederer, Emma;Levin, Michael
迄今为止，机器人群是由人造材料建造的。运动的生物结构已经从生长在精确成形的支架上的肌肉细胞中产生。然而，利用涌现的自组织和功能可塑性成为一个自我导向的生活机器仍然是一个重大挑战。我们在这里报告的方法，在体外生物机器人从青蛙（非洲爪蟾）细胞。这些异种机器人通过其表面上的纤毛表现出协调的运动。这些纤毛通过正常组织模式产生，不需要复杂的构建方法或基因组编辑，使生产适合高通量项目。生物机器人是由细胞自组织产生的，不需要支架或微缩打印;两栖动物细胞在自组装过程中非常适合手术，遗传，化学和光学刺激。我们表明，异种机器人可以以不同的方式导航水环境，损伤后愈合，并显示紧急群体行为。我们构建了一个计算模型来预测有用的集体行为，可以引起从一个异种机器人群。此外，我们提供了一个可写的分子存储器的原理证明，使用光转换蛋白质，可以记录暴露于特定波长的光。总之，这些结果引入了一个平台，可用于研究自组装，群体行为和合成生物工程的许多方面，并为生物医学和环境中的许多实际应用提供多功能的软体生物机器。
Robot swarms have, to date, been constructed from artificial materials. Motile biological constructs have been created from muscle cells grown on precisely shaped scaffolds. However, the exploitation of emergent self-organization and functional plasticity into a self-directed living machine has remained a major challenge. We report here a method for generation of in vitro biological robots from frog (Xenopus laevis) cells. These xenobots exhibit coordinated locomotion via cilia present on their surface. These cilia arise through normal tissue patterning and do not require complicated construction methods or genomic editing, making production amenable to high-throughput projects. The biological robots arise by cellular self-organization and do not require scaffolds or microprinting; the amphibian cells are highly amenable to surgical, genetic, chemical, and optical stimulation during the self-assembly process. We show that the xenobots can navigate aqueous environments in diverse ways, heal after damage, and show emergent group behaviors. We constructed a computational model to predict useful collective behaviors that can be elicited from a xenobot swarm. In addition, we provide proof of principle for a writable molecular memory using a photoconvertible protein that can record exposure to a specific wavelength of light. Together, these results introduce a platform that can be used to study many aspects of self-assembly, swarm behavior, and synthetic bioengineering, as well as provide versatile, soft-body living machines for numerous practical applications in biomedicine and the environment.