HP1 controls telomere capping, telomere elongation, and telomere silencing by two different mechanisms in Drosophila
HP1 controls telomere capping, telomere elongation, and telomere silencing by two different mechanisms in Drosophila
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DOI：
10.1016/j.molcel.2004.06.036
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发表时间：
2004-08-13
期刊：
影响因子：
16
通讯作者：
Pimpinelli, S
中科院分区：
文献类型：
--
作者：
Perrini, B;Piacentini, L;Pimpinelli, S
HP 1是一种保守的染色体蛋白，首先在果蝇中发现，它主要与许多生物的异染色质相关。最近，它已被证明，HP 1是必需的端粒帽，端粒延长，转录抑制端粒序列。几项研究提出了一种不同物种中异染色质形成和表观遗传基因沉默的模型，该模型基于组蛋白甲基转移酶（HMTases）、组蛋白H3在赖氨酸9处甲基化（H3-MeK 9）和HP 1染色体结构域之间的相互作用。该模型已被扩展到HP 1端粒定位的数据显示，H3-MeK 9存在于所有的端粒。在这里，我们测试了这个模型，我们发现HP 1的加帽功能是由于它直接结合端粒DNA，而端粒序列的沉默和端粒延长是由于它与H3-MeK 9的相互作用。
HP1 is a conserved chromosomal protein, first discovered in Drosophila, which is predominantly associated with the heterochromatin of many organisms. Recently, it has been shown that HP1 is required for telomere capping, telomere elongation, and transcriptional repression of telomeric sequences. Several studies have suggested a model for heterochromatin formation and epigenetic gene silencing in different species that is based on interactions among histone methyltransferases (HMTases), histone H3 methylated at lysine 9 (H3-MeK9), and the HP1 chromodomain. This model has been extended to HP1 telomeric localization by data showing that H3-MeK9 is present at all of the telomeres. Here, we tested this model, and we found that the capping function of HP1 is due to its direct binding to telomeric DNA, while the silencing of telomeric sequences and telomere elongation is due to its interaction with H3-MeK9.