Direct observation of nanomechanical properties of chromatin in living cells
Direct observation of nanomechanical properties of chromatin in living cells
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DOI：
10.1021/nl070603
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发表时间：
2007-05-01
期刊：
影响因子：
10.8
通讯作者：
Kanger, Johannes S.
中科院分区：
文献类型：
--
作者：
de Vries, Anthony H. B.;Krenn, Bea E.;Kanger, Johannes S.
使用磁镊子对纳米磁性颗粒的精确操纵使人们对细胞质的流变性有了更深入的了解。我们介绍了使用这种方法研究细胞核的纳米力学的第一个结果。使用定制设计的微型磁镊子仪器，我们可以获得足够高的磁力，从而能够在纳米尺度上应用和测量内部核结构的受控变形。我们精确测量活的HeLa细胞细胞核内的弹性和粘度。测量到的相对于细胞质的高杨氏模数值(Y=2.5×10(2)Pa.)可以用一个大尺度的体内染色质结构模型来解释。
Precise manipulation of nanometer-sized magnetic particles using magnetic tweezers has yielded insights into the rheology of the cell cytoplasm. We present first results using this approach to study the nanomechanics of the cell nucleus. Using a custom-designed micro-magnetic-tweezers instrument, we can achieve sufficiently high magnetic forces enabling the application and measurement of controlled distortion of the internal nuclear structure on the nanometer scale. We precisely measure the elasticity and viscosity inside the nucleus of living HeLa cells. The high value of the Young's modulus (Y = 2.5 x 10(2) Pa) measured relative to the cytoplasm is explained by a large-scale model for in vivo chromatin structure using a polymer network model.