Modulation of esophageal contractions by distension in vitro.
Modulation of esophageal contractions by distension in vitro.
复制标题
通过体外扩张调节食管收缩。
DOI：
10.1007/bf01536324
复制
发表时间：
1989
影响因子：
3.1
通讯作者：
Schulze-Delrieu,K
中科院分区：
文献类型：
--
作者：
Ren,J;Schulze-Delrieu,K
食管体的内在神经的刺激在刺激结束后导致环行肌的抽搐（食管关闭响应），并且由于近端和远端食管之间的关闭响应的开始的延迟梯度，抽搐在远端方向上传播。我们研究了局部输入可以通过内在机制调节基本关闭响应的可能性，并使其更接近于在完整动物（食管扩张）的食管中移动推注的顺序环收缩。从负鼠中取出食管（上至主动脉弓，下至胃食管交界处），并在36° C下悬浮于含有2升Krebs溶液的器官浴中。通过LES上方2、4和6 cm处食管浆膜表面上的力传感器记录食管的机械活动。近端食管的壁内神经被电脉冲刺激，有和没有通过管腔球囊膨胀的食管扩张。球囊扩张增加了远端食管的关闭反应的潜伏期，从而降低了环形肌肉收缩通过食管传播的速度。此外，球囊扩张增加了食管环形肌收缩的幅度和持续时间（关闭和打开响应），并降低了纵向肌肉收缩（持续时间响应）和LES响应（松弛响应）的幅度。食管收缩反应的类似变化是由径向拉伸的开放准备食管粘膜已被删除。本研究结果表明，壁内机制可以调节的收缩模式的离体食管平滑肌。观察到的变化使得径向拉伸可能是将食管平滑肌的固有关闭响应转变为在完整动物中观察到的食管食团转运的顺序环收缩的重要因素。
Stimulation of the intrinsic nerves of the esophageal body leads to a twitch of the circular muscle after the end of the stimulation (the esophageal off-response), and the twitch spreads in the distal direction because of a latency gradient in the onset of the off-response between the proximal and the distal esophagus. We investigated the possibility that local input can modulate the basic off-response through intrinsic mechanisms and make it resemble more closely the sequential ring contractions that move boluses in the esophagus of intact animals (esophageal peristalsis). The esophagus up to the aortic arch and down to the gastroesophageal junction was removed from opossums and suspended in an organ bath containing 2 liters of Krebs solution at 36° C. The mechanical activity of the esophagus was recorded by force transducers on the serosal surface of the esophagus 2, 4, and 6 cm above the LES. The intramural nerves of the proximal esophagus were stimulated by electrical pulses with and without distension of the esophagus by inflation of a luminal balloon. Balloon distension increased the latency of the off-response in the distal esophagus, thereby reducing the velocity at which the circular muscle contraction spread through the esophagus. In addition, balloon distension increased the amplitude and the duration of the esophageal circular muscle contraction (both off- and on-responses), and decreased the amplitude of the longitudinal muscle contraction (duration response) and LES response (relaxation response). Similar changes in the esophageal contraction responses were produced by radial stretch of an open preparation of the esophagus from which the mucosa had been removed. The present results demonstrate that intramural mechanisms can modulate the contraction pattern of the isolated esophageal smooth muscle. The observed changes make it likely that radial stretch is an important factor in turning the intrinsic off-response of the esophageal smooth muscle into the sequential ring contraction observed with esophageal bolus transport in the intact animal.