Identification of pulmonary edema in forensic autopsy cases of fatal anaphylactic shock using Fourier transform infrared microspectroscopy
Identification of pulmonary edema in forensic autopsy cases of fatal anaphylactic shock using Fourier transform infrared microspectroscopy
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傅里叶变换红外显微光谱法鉴别致死性过敏性休克法医尸检中肺水肿
DOI：
10.1007/s00414-017-1721-4
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发表时间：
2018
影响因子：
2.1
通讯作者：
Huang Ping
中科院分区：
文献类型：
--
作者：
Lin Hancheng;Luo Yiwen;Wang Lei;Deng Kaifei;Sun Qiran;Fang Ruoxi;Wei Xin;Zha Shuai;Wang Zhenyuan;Huang Ping
过敏反应是一种快速的过敏反应，可能导致猝死。目前，过敏性休克的尸检诊断有时很困难，通常通过排除来实现。本研究的目的是探讨傅立叶变换红外（FTIR）显微光谱结合模式识别方法是否可以补充传统方法，并提供一个更准确的死后诊断致命的过敏性休克。首先，光谱峰面积分析结果显示，致死性过敏性休克组的肺水肿液比对照组更富含蛋白质成分，后者包括机械性窒息、脑损伤和急性心源性死亡。随后进行主成分分析（PCA），结果显示过敏性休克组比对照组含有更多的转角和α-螺旋蛋白质结构，而富含酪氨酸的蛋白质含量较少。最后，偏最小二乘判别分析（PLS-DA）模型结合变量选择方法称为遗传算法（GA）的建立，并表现出良好的分离这两组之间。这项初步研究表明，傅立叶变换红外显微光谱有可能成为一个有效的援助，死后诊断致命的过敏性休克。
Anaphylaxis is a rapid allergic reaction that may cause sudden death. Currently, postmortem diagnosis of anaphylactic shock is sometimes difficult and often achieved through exclusion. The aim of our study was to investigate whether Fourier transform infrared (FTIR) microspectroscopy combined with pattern recognition methods would be complementary to traditional methods and provide a more accurate postmortem diagnosis of fatal anaphylactic shock. First, the results of spectral peak area analysis showed that the pulmonary edema fluid of the fatal anaphylactic shock group was richer in protein components than the control group, which included mechanical asphyxia, brain injury, and acute cardiac death. Subsequently, principle component analysis (PCA) was performed and showed that the anaphylactic shock group contained more turn and α-helix protein structures as well as less tyrosine-rich proteins than the control group. Ultimately, a partial least-square discriminant analysis (PLS-DA) model combined with a variables selection method called the genetic algorithm (GA) was built and demonstrated good separation between these two groups. This pilot study demonstrates that FTIR microspectroscopy has the potential to be an effective aid for postmortem diagnosis of fatal anaphylactic shock.