Photopatterning of self-assembled poly (ethylene) glycol monolayer for neuronal network fabrication
Photopatterning of self-assembled poly (ethylene) glycol monolayer for neuronal network fabrication
复制标题
用于神经元网络制造的自组装聚乙二醇单层的光图案化
DOI：
10.1016/j.jneumeth.2012.12.020
复制
发表时间：
2013-03-15
影响因子：
3
通讯作者：
Xu, Yuansen
中科院分区：
文献类型：
--
作者：
Cheng, Ji;Zhu, Geng;Xu, Yuansen
培养单个神经元并指导它们在功能接口上的连接的能力为研究神经元网络中的信息处理提供了一个平台。在微电极阵列（MEAs）上设计有序神经网络用于记录神经元电活动的方法有很多。然而，到目前为止，还没有一种方法能够同时提供高分辨率的神经元模式和低阻抗的微电极。为了实现这一目标，我们采用了一种化学气相沉积、无污染的聚乙二醇（PEG）自组装单层材料，在MEAs上提供细胞排斥背景。利用光刻技术和等离子体刻蚀聚乙二醇单层，在MEAs上制备了不同的图案。在整个过程中没有观察到电极性能下降。分离的皮质神经元在改良的MEAs上培养，其模式维持3周以上。记录自发性和诱发性神经元活动。所有的结果都表明，这种表面工程策略可以成功地在mea上绘制神经元的图案，并且对芯片上定义的神经元网络的信息处理的未来研究很有用。(c) 2013 Elsevier B.V.版权所有
The ability to culture individual neurons and direct their connections on functional interfaces provides a platform for investigating information processing in neuronal networks. Numerous methods have been used to design ordered neuronal networks on microelectrode arrays (MEAs) for neuronal electrical activities recording. However, so far, no method has been implemented, which simultaneously provides high-resolution neuronal patterns and low-impedance microelectrode. To achieve this goal, we employed a chemical vapor-deposited, non-fouling poly (ethylene) glycol (PEG) self-assembled monolayer to provide a cell repellant background on the MEAs. Photolithography, together with plasma etching of the PEG mono-layer, was used to fabricate different patterns on MEAs. No electrode performance degradation was observed after the whole process. Dissociated cortical neurons were cultured on the modified MEAs, and the patterns were maintained for more than 3 weeks. Spontaneous and evoked neuronal activities were recorded. All of the results demonstrate this surface engineering strategy allows successful patterning of neurons on MEAs, and is useful for future studies of information processing in defined neuronal networks on a chip. (c) 2013 Elsevier B.V. All rights reserved.