Low venular shear rates promote leukocyte-dependent recruitment of adherent platelets
Low venular shear rates promote leukocyte-dependent recruitment of adherent platelets
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DOI：
10.1152/ajpgi.00303.2002
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发表时间：
2003-01-01
影响因子：
4.5
通讯作者：
Granger, DN
中科院分区：
文献类型：
--
作者：
Russell, J;Cooper, D;Granger, DN
小静脉剪切率的降低对血小板-内皮（P/E）细胞粘附的影响以前尚未得到解决。本研究的目的是确定在P/E细胞粘附的小静脉剪切率的降低的影响，并确定在低剪切率的P/E细胞粘附和白细胞-内皮（L/E）细胞粘附的相互依赖性。活体视频显微镜用于定量大鼠肠系膜微静脉中P/E和L/E细胞粘附，暴露于118 +/- 9和835 +/- 44 s（-1）之间的剪切速率。通过快速、分级的抽血改变毛细血管后微静脉的剪切速率，而不回输流出的血液。将剪切速率从>600 s（-1）降低到
The influence of reductions in venular shear rate on platelet-endothelial (P/E) cell adhesion has not been previously addressed. The objectives of this study were to define the effects of reductions in venular shear rate on P/E cell adhesion and to determine the interdependence of P/E cell adhesion and leukocyte-endothelial (L/E) cell adhesion at low shear rates. Intravital videomicroscopy was used to quantify P/E and L/E cell adhesion in rat mesenteric venules exposed to shear rates ranging between 118 +/- 9 and 835 +/- 44 s(-1). Shear rate was altered in postcapillary venules by rapid, graded blood withdrawal, without retransfusion of shed blood. Reducing shear rate from >600 s(-1) to