Monitoring pH and dissolved oxygen in mammalian cell culture using optical sensors
Monitoring pH and dissolved oxygen in mammalian cell culture using optical sensors
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DOI：
10.1007/s10616-008-9160-1
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发表时间：
2008-07-01
期刊：
影响因子：
2.2
通讯作者：
Al-Rubeai, Mohamed
中科院分区：
文献类型：
--
作者：
Naciri, Mariam;Kuystermans, Darrin;Al-Rubeai, Mohamed
在这里，我们研究了在哺乳动物细胞培养中对过程控制至关重要的两个参数：溶解氧(DO(2))和用荧光传感器测量的pH，从而可以在不去除或破坏培养过程中的细胞的情况下研究细胞在培养中的代谢状态。NS0和CHO两种细胞系在24孔板中以不同的血清浓度批量培养，传感器安装在每孔的底部。数据表明，荧光探针测得的DO(2)和pH数据与细胞的生长和死亡特性之间有很好的相关性。该方法提供了一种高通量在线多参数哺乳动物细胞活性分析方法。
Here, we have studied two parameters critical to process control in mammalian cell culture; dissolved oxygen (dO(2)) and pH, measured with fluorescent sensors thus allowing the study of the metabolic state of cells in culture without removing or damaging cells during cultivation. Two cell lines, namely, NS0 and CHO were batch-grown in 24-well plates at different serum concentrations with the sensors implemented in the bottom of each well. The data showed a good relationship between the dO(2) and pH data obtained from fluorescent probes and the growth and death characteristics of cells. The method has provided a high throughput on-line multi-parametric analysis of mammalian cell cellular activity.