Adult pancreatic stem/progenitor cells spontaneously differentiate in vitro into multiple cell lineages and form teratoma-like structures
Adult pancreatic stem/progenitor cells spontaneously differentiate in vitro into multiple cell lineages and form teratoma-like structures
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DOI：
10.1016/j.aanat.2006.07.012
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发表时间：
2006-11-01
影响因子：
2.2
通讯作者：
Wedel, Thilo
中科院分区：
文献类型：
--
作者：
Krusea, Charli;Kajahn, Jennifer;Wedel, Thilo
胰腺细胞具有显著的自我更新和多系分化潜能。我们在此提出了来自成年大鼠胰腺外分泌部分的干细胞/祖细胞的综合特征。使用来自单个菌落或甚至单细胞克隆的纯化细胞，我们特别证明：(i)细胞含有典型的。干细胞/祖细胞标志物碱性磷酸酶、SSEA-1、Oct-4、CD9、Nestin、Pax6、CD44、甲胎蛋白和Brachyury，经免疫细胞化学和RT-PCR证实；（ii）细胞在体外具有分化为所有三种胚层谱系的潜力；（iii）克隆分析显示，即使是来自单个细胞的细胞系也具有干细胞/祖细胞的特性，如自我更新和自发分化为各种细胞系；（iv）细胞在体外具有形成三维畸胎瘤样结构的倾向，其中包含不同谱系的细胞；(5)外部刺激可以激活某些细胞类型的生成。例如，用维甲酸处理的细胞显示α -平滑肌肌动蛋白的表达增加。这些结果提示，胰腺等外分泌腺可能是成体干细胞/祖细胞的潜在来源，适用于退行性疾病的细胞治疗。(c) 2006 Elsevier GmbH版权所有。
Cells isolated from pancreas have a remarkable potential for self-renewal and multilineage differentiation. We here present a comprehensive characterisation of stem/progenitor cells derived from exocrine parts of the adult rat pancreas. Using purified cells from either single colonies or even single-cell clones, we specifically demonstrate: (i) the cells contain the typical. stem/progenitor cell markers alkaline phophatase, SSEA-1, Oct-4, CD9, Nestin, Pax6, CD44, alpha-Fetoprotein and Brachyury, demonstrated by immunocytochemistry and RT-PCR; (ii) the cells have the potential to differentiate into lineages of all three germ layers in vitro; (iii) a clonal analysis revealed that even cell lines derived from a single cell have stem/progenitor cell properties such as self-renewal and spontaneous differentiation into various cell lineages; (iv) the cells have the propensity to form three-dimensional, teratoma-like structures in vitro, which contain cells of different lineages; and (v) external stimuli can activate the generation of certain cell types. For instance, cells treated with retinoic acid show an increased expression of alpha-smooth muscle actin.These results suggest that exocrine glands, such as pancreas may be a potential source of adult stem/progenitor cells, suitable for cell therapy of degenerative diseases . (c) 2006 Elsevier GmbH. All rights reserved.