Detection of suppressed maturation of the human COQ5 protein in the mitochondria following mitochondrial uncoupling by an antibody recognizing both precursor and mature forms of COQ5
Detection of suppressed maturation of the human COQ5 protein in the mitochondria following mitochondrial uncoupling by an antibody recognizing both precursor and mature forms of COQ5
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DOI：
10.1016/j.mito.2013.01.007
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发表时间：
2013-03-01
期刊：
影响因子：
4.4
通讯作者：
Yen, Hsiu-Chuan
中科院分区：
文献类型：
--
作者：
Chen, Shih-Wei;Liu, Chia-Chi;Yen, Hsiu-Chuan
酵母Coq5p是辅酶Q（6）（CoQ（6））生物合成所必需的，但其人类同系物尚未研究。我们在天然条件下纯化了可溶性重组人COQ5蛋白，并产生了识别COQ5前体和成熟形式的抗体。用该抗体证明了143 B细胞中成熟形式的线粒体定位。此外，抑制辅酶Q（10）水平的化学解偶联剂下调了成熟形式，但增加了辅酶Q 5的前体形式。敲除COQ5基因降低CoQ（10）水平的结果进一步表明了COQ5在CoQ生物合成中的关键作用（10）。(C)2013 Elsevier B.V.和线粒体研究学会。All rights reserved.
Yeast Coq5p is required for the biosynthesis of coenzyme Q(6) (CoQ(6)), but its human homolog has not been studied. We purified soluble recombinant human COQ5 protein under native conditions and generated an antibody recognizing both precursor and mature forms of COQ5. Mitochondrial localization of the mature form in 143B cells was demonstrated with this antibody. Moreover, a chemical uncoupler in a dose that suppressed CoQ(10) levels downregulated the mature form but augmented the precursor form of COQ5. The results that knockdown of the COQ5 gene reduced CoQ(10) levels further indicated the critical role of COQ5 in the biosynthesis of CoQ(10). (C) 2013 Elsevier B.V. and Mitochondria Research Society. All rights reserved.