NMR analysis of rhodopsin-transducin interactions.
NMR analysis of rhodopsin-transducin interactions.
复制标题
视紫质-转导蛋白相互作用的核磁共振分析。
DOI：
10.1016/j.visres.2006.07.024
复制
发表时间：
2006
期刊：
影响因子：
1.8
通讯作者：
Abdulaev,NG
中科院分区：
文献类型：
--
作者：
Ridge,KD;Marino,JP;Ngo,T;Ramon,E;Brabazon,DM;Abdulaev,NG
Heterotrimeric G-protein activation by an agonist-stimulated G-protein coupled receptor (R∗) requires the propagation of structural signals from the receptor interacting surfaces to the guanine nucleotide-binding pocket. Employing high-resolution NMR methods, we are probing heterotrimer-associated and rhodopsin-stimulated changes in an isotope-labeled G-protein α-subunit (Gα). A key aspect of the work involves the trapping and interrogation of discrete R∗-bound conformations of Gα. Our results demonstrate that functionally important changes in Gαstructure and dynamics can be detected and characterized by NMR, enabling the generation of robust models for the global and local structural changes accompanying signal transfer from R∗to the G-protein.
