RIM4 encodes a meiotic activator required for early events of meiosis in Saccharomyces cerevisiae
RIM4 encodes a meiotic activator required for early events of meiosis in Saccharomyces cerevisiae
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DOI：
10.1007/s004380100571
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发表时间：
2001-11-01
影响因子：
3.1
通讯作者：
Saunders, WS
中科院分区：
文献类型：
--
作者：
Deng, C;Saunders, WS
先前发现RIM4是酿酒酵母减数分裂基因表达的IME1和IME2依赖途径所必需的。我们现在证明，RIM4也需要减数分裂和重组。此外，rim4 Delta突变体显示减数分裂前DNA合成的缺陷，这可以通过缺失编码Cdk抑制剂的SIC1基因来抑制。RIM 4的表达在减数分裂早期被诱导，并且依赖于IME 1而不是IME 2。事实上，RIM4本身对于IME2的减数分裂表达是必需的。这些结果表明，RIM 4是上位的IME 2，并需要在孢子形成过程中的多个步骤。与这种解释一致，RIM 4的过表达诱导丰富培养基中低水平的孢子形成。
RIM4 was previously found to be required for both the IME1- and IME2-dependent pathways of meiotic gene expression in Saccharomyces cerevisiae. We now demonstrate that RIM4 is also required for meiotic division and recombination. Furthermore, rim4 Delta mutants show defects in premeiotic DNA synthesis, which can be suppressed by deletion of the SIC1 gene, which encodes a Cdk inhibitor. Expression of RIM4 is induced early in meiosis, and is dependent on IME1 but not IME2. Indeed, RIM4 itself is essential for the meiotic expression of IME2. These results suggest that RIM4 is epistatic to IME2, and is required for multiple steps during sporulation. In agreement with this interpretation, overexpression of RIM4 induces low levels of sporulation in rich medium.