Carbohydrate metabolism and activity variation of related enzymes during the exchanging role of bulb source and sink of oriental hybrid lily‘Sorbonne’
Carbohydrate metabolism and activity variation of related enzymes during the exchanging role of bulb source and sink of oriental hybrid lily‘Sorbonne’
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发表时间：
2013
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通讯作者：
Wu Sha-sh
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作者：
Wu Sha-sh
采用紫外分光光度法测定了东方杂交百合'索邦'的碳水化合物含量及其相关酶活性，并利用SPASS软件分析了它们之间的相关性。结果表明：从定植到花蕾发育至3 cm长，鳞茎中、外鳞片淀粉含量呈下降趋势，说明鳞茎在此过程中起源作用;开花后，鳞茎中、外鳞片淀粉代谢呈相反方向，说明鳞茎起源库复合体作用，此后鳞茎成为库积累养分。此外，外鳞和中鳞的蔗糖、蔗糖合成酶（SS）和蔗糖磷酸合成酶（SPS）均呈显著正相关，而淀粉含量与SS和SPS呈显著负相关，但均未达到显著水平。此外，除中间鳞片的可溶性淀粉合成酶（SSS）与淀粉含量呈低度负相关外，外、中鳞片的淀粉含量与腺苷二磷酸葡萄糖焦磷酸化酶（AGP 3）、淀粉合成酶（SSS和颗粒结合型淀粉合成酶GBSS）均呈显著正相关，尤其是外鳞片的淀粉含量与SSS和GBSS呈高度正相关。与淀粉合成有关的三种酶与蔗糖含量呈负相关。淀粉合成相关酶的活性可作为百合种球源库功能的重要参考。SSS和GBSS活性对百合鳞茎库强度的决定作用更大，可作为百合鳞茎库强度的指标。
UV spectrophotometer methods were used to assay the carbohydrate contents and activities of related enzymes of oriental hybrid lily ‘Sorbonne',and their correlations were analyzed using SPASS. The results showed that: the content of starch in outer and middle scales showed a downward trend from planting to flower bud developing to 3 cm in length,which indicated that the bulb served as source during this process; thereafter to anthesis,the metabolism of starch in the outer and middle scales showed the opposite directions,indicating that the bulbs served as the source-sink complex; from then on,the bulbs became sink to accumulate nutrients. Besides,sucrose and sucrose synthesis enzymes( SS) and sucrose phosphate synthase( SPS) in outer and middle scales showed positive correlations respectively,while starch content and SS and SPS showed negative correlations,all of which were not statistically significant. Furthermore,except that the soluble starch synthase( SSS) and starch content in middle scales showed a low negative correlation,the starch content and the adenosine diphosphate glucose pyrophosphorylase( AGPase),and starch synthase( SSS and granule-bound starch synthase GBSS) in outer and middle scales showed positive correlation,especially the correlation between starch content and SSS and GBSS in the outer scales,which were high positive and significant. Three enzymes relating to starch synthesis showed negative correlation with sucrose content. The activities of enzymes relating to starch synthesis can serve as important references for the source or sink function of lily bulbs. The activities of SSS and GBSS played more significant roles in deciding the sink strength of lily bulbs,which can be used as indicators for the sink strength of lily bulbs.