INTRAERYTHROCYTIC MULTIPLICATION OF THEILERIA-PARVA INVITRO - AN ULTRASTRUCTURAL-STUDY
INTRAERYTHROCYTIC MULTIPLICATION OF THEILERIA-PARVA INVITRO - AN ULTRASTRUCTURAL-STUDY
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DOI：
10.1016/0020-7519(86)90047-0
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发表时间：
1986-06-01
影响因子：
4
通讯作者：
BROWN, CGD
中科院分区：
文献类型：
--
作者：
CONRAD, PA;DENHAM, D;BROWN, CGD
从肯尼亚Muguga分离出一株细小伊氏芽孢杆菌（Theileria parva），在短期固定的甲状腺细胞培养中保持其梨质，并通过光镜和电镜研究其增殖模式。在体外24小时内观察到单个梨质分裂为4个红细胞内分裂子。经过8天的培养，10-20%的被寄生红细胞在体外增殖后含有四联体分裂子。电子显微镜显示，红细胞内增殖是通过分裂过程发生的，从最初形成的异构体和电子致密的细胞质斑块，到核分裂，最后分离出最多四个分化的分生子。体外红细胞分裂体形成的分裂子具有与小孢子虫淋巴细胞分裂体产生的分裂子相同的超微结构特征。
Piroplasms of a Theileria parva isolate from Muguga, Kenya were maintained in short-term stationary erthyrocyte cultures and the mode of multiplication studied by light and electron microscopy. Division of single piroplasms into four intraerythrocyte merozoites was observed within the first 24 h in vitro. After 8 days of cultivation, 10-20% of the parasitised erythrocytes contained quadruplet merozoite forms resulting from multiplication in vitro. Electronmicrographs showed that intraerythrocytic multiplication occured by a schizogonous process that proceeded from the initial formation of rhoptries and electron-dense cytoplasmic plaques beneath the parasite''s plasmalemmal membrane, to nuclear division and the final separation of a maximum of four differentiated merozoites. The merozoites formed by intraerythrocytic schizogony in vitro had ultrastructural features identical to merozoites produced by intralymphocytic schizonts of T. parva.