Preparation of gardenia red pigment and its antineoplastic activity in multiple tumor cells
Preparation of gardenia red pigment and its antineoplastic activity in multiple tumor cells
复制标题
栀子红色素的制备及其对多种肿瘤细胞的抗肿瘤活性
DOI：
10.1016/j.fbio.2020.100582
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发表时间：
2020-06-01
期刊：
影响因子：
5.2
通讯作者：
Sun, Baoguo
中科院分区：
文献类型：
--
作者：
Hao, Shuai;Wang, Jing;Sun, Baoguo
栀子红色素，也称为京尼平红，是一种天然食品着色剂，用于食品工业。它是由氨基酸与京尼平、京尼平苷酸（GPA）和京尼平苷的糖苷配基反应而产生的，其在栀子黄生产的副产物中含量丰富。但由于杂质含量高，栀子红色素生产表现出较低的色价和纯度，限制了其应用。本研究以栀子黄生产副产物为原料，对栀子红色素的制备和纯化进行了研究。京尼平苷酸和京尼平与组氨酸反应生成栀子红。通过优化得到了色价为117、纯度为91.1%的栀子红色素。体外生物活性试验表明，栀子红色素对H1299、A375和MCF-7肿瘤细胞具有较强的杀伤活性。本研究优化了栀子红的制备工艺，并揭示了栀子红的潜在功能。
Gardenia red pigment, also referred to genipin red, is a natural food colorant used in the food industry. It is produced by the reaction of amino acids with genipin, the aglycon of geniposide acid (GPA) and geniposide, which is abundant in the by-products of gardenia yellow production. However, due to the high content of impurities, gardenia red pigment production shows low color value and purity, which limits its application. In this study, the preparation and purification of gardenia red pigments using by-products of gardenia yellow production as raw materials were investigated. Purified geniposidic acid and genipin were obtained and reacted with His to give gardenia red. After optimization, gardenia red with high color value (117) and purity (91.1%) was obtained. Furthermore, the in vitro biological functional assays suggested that gardenia red pigments showed high antineoplastic activity with H1299, A375 and MCF-7 cancer cells. This study has developed an optimized preparation process and shown potential functions of gardenia red.