A core-shell polymeric-inorganic hybrid nanocomposite system for MRI-visible gene delivery application in cancer immunotherapy
A core-shell polymeric-inorganic hybrid nanocomposite system for MRI-visible gene delivery application in cancer immunotherapy
复制标题
用于癌症免疫治疗中 MRI 可见基因传递应用的核壳聚合物-无机杂化纳米复合材料系统
DOI：
10.1016/j.jiec.2019.03.039
复制
发表时间：
2019-08-25
影响因子：
6.1
通讯作者：
Ai, Hua
中科院分区：
文献类型：
--
作者：
Cai, Jiali;Chen, Guochuang;Ai, Hua
由于肿瘤的免疫逃逸机制，采用双特异性抗体等策略来辅助免疫治疗。本文制备了可见的聚合物-无机杂合基因载体，将编码抗EpCAM/抗CD3的小环DNA递送至正常细胞中，从而使这些转染细胞有效地分泌具有生物活性的双特异性抗体。在实验中，该复合系统表现出优异的生物相容性、高转染效率和超灵敏的成像能力。此外，转染的细胞可以在MR成像系统下检测到，并且基因产物表现出突出的免疫效果。总之，这种纳米复合材料在基因传递和非侵入性磁共振成像方面表现出协同优势。 (C) 2019 韩国产业工程化学学会。由 Elsevier B.V. 出版。保留所有权利。
Due to the tumor immune escape mechanism, strategies like the utilization of bispecific antibodies were used to assist immunotherapy. Herein a visible polymer-inorganic hybrid gene vehicle had been prepared to deliver anti-EpCAM/anti-CD3 encoding minicircle DNA into normal cells thus these transfected cells effectively secreted bispecific antibody with bioactivity. In the experiments, this compound system exhibited excellent biocompatibility, high transfection efficiency and ultrasensitive imaging capacity. Furthermore, the transfected cells could be detected under the MR imaging system and the gene product showed an outstanding immune effect. In conclusion, this nanocomposite showed synergistic advantages in gene delivery and non-invasive MR imaging. (C) 2019 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.