Immunohistochemical analysis of inflammatory limbal conjunctiva adjacent to Mooren's ulcer
Immunohistochemical analysis of inflammatory limbal conjunctiva adjacent to Mooren's ulcer
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DOI：
10.1136/bjophthalmol-2012-302631
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发表时间：
2013-01
影响因子：
4.1
通讯作者：
K. Shinomiya;M. Ueta;C. Sotozono;T. Inatomi;N. Yokoi;N. Koizumi;S. Kinoshita
中科院分区：
文献类型：
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作者：
K. Shinomiya;M. Ueta;C. Sotozono;T. Inatomi;N. Yokoi;N. Koizumi;S. Kinoshita
目的探讨蚕蚀性角膜溃疡周边结膜组织浸润细胞的特征。方法对4例蚕蚀性角膜溃疡患者的4只眼进行手术治疗。将患者切除的结膜组织包埋并冷冻。然后对组织切片进行H&E和免疫组织化学染色。对结膜组织进行染色切片观察，并对结膜组织中浸润细胞的特征进行病理检查。结果所有患者的结膜黏膜下结缔组织均可见炎性细胞浸润。免疫组化分析显示结膜粘膜下层有炎性细胞浸润，主要由CD3阳性和CD45RO阳性细胞组成。这些细胞中的一些也显示出与CD4的阳性反应性，但极少数细胞显示出与CD8的阳性反应性。此外，在少数情况下，指示CD68阳性的细胞浸润频繁。结论4例蚕蚀性角膜溃疡患者角膜溃疡周边结膜组织粘膜下层浸润细胞主要由辅助性T淋巴细胞和巨噬细胞组成。我们的研究结果表明，辅助性T细胞和巨噬细胞有助于蚕蚀性溃疡的发病机制。
Background/aims To examine the characteristics of infiltrating cells in conjunctival tissues adjacent to the peripheral corneal ulcers of Mooren's ulcer. Methods This study involved four eyes of four patients with Mooren's ulcer and who were considered to be in need of surgical treatment. The patients’ resected conjunctival tissues were embedded and frozen. The tissue sections were then subjected to H&E and immunohistochemical staining. The stained sections were observed and the characteristics of the infiltrating cells in the conjunctival tissues were pathologically examined. Results In all patients, infiltration of inflammatory cells was observed in the submucosal connective tissue of the conjunctiva. Immunohistochemical analysis revealed inflammatory cell infiltration into the submucosal layer of the conjunctiva that was mainly composed of CD3-positive and CD45RO-positive cells. Some of these cells also showed positive reactivity with CD4, yet very few cells showed positive reactivity with CD8. In addition, infiltration of the cells indicating CD68 positivity was frequent in a few cases. Conclusions In the four Mooren's ulcer cases, infiltrating cells in the submucosa of the conjunctival tissues adjacent to the ulcerative cornea were found to be mainly composed of helper T lymphocytes and macrophages. Our results show that helper T cells and macrophages contribute to the pathogenesis of Mooren's ulcer.