Inactivation of the global regulator LaeA in Monascus ruber results in a species-dependent response in sporulation and secondary metabolism
Inactivation of the global regulator LaeA in Monascus ruber results in a species-dependent response in sporulation and secondary metabolism
复制标题
红曲霉中全局调节因子 LaeA 的失活导致孢子形成和次生代谢的物种依赖性反应
DOI：
10.1016/j.funbio.2015.10.008
复制
发表时间：
2016-03-01
期刊：
影响因子：
2.5
通讯作者：
Chen, Fusheng
中科院分区：
文献类型：
--
作者：
Liu, Qingpei;Cai, Li;Chen, Fusheng
核调节因子LaeA已被证明在全球范围内控制真菌的发育和次生代谢，但其功能可能是物种依赖的，尽管其氨基酸序列在许多真菌中都很保守。本研究鉴定了红曲霉M7 （MrLaeA）中的LaeA，并验证了其在调节生长、产孢和次生代谢中的作用。结果表明，所选MrlaeA敲除突变体Mr Delta laeA-22的径向生长速度明显快于亲本菌株M. ruber M7，生长过程中出现异常菌落表型，空生菌丝更丰富。有趣的是，Mr Delta laeA22菌株的分生孢子产量约为M. ruber M7的三倍，但在Mr Delta laeA-22菌株中未观察到子囊孢子。此外，与M. ruber M7相比，Mr Delta laeA-22的多种次生代谢物产量显著降低，尤其是6种红曲霉色素和柑桔素的产量。同时，选择的MrlaeA互补菌株（Mr Delta laeA::laeA-45）的产孢能力和次生代谢能力几乎恢复了亲本菌株的水平。这些结果表明，MrLaeA不仅调控橡胶分枝杆菌的次生代谢，还调控无性和有性分化，但其调控的某些方面似乎不同于其他真菌。(C) 2015英国真菌学学会。Elsevier Ltd.出版。版权所有。
The nuclear regulator LaeA has been proven to globally govern fungal development and secondary metabolism, but its function may be species-dependent, even though its amino acid sequences are well conserved in numerous fungi. Herein we identified the LaeA in Monascus ruber M7 (MrLaeA), and verified its role to mediate growth, sporulation and secondary metabolism. Results showed that the radial growth rate of the selected MrlaeA knock-out mutant (Mr Delta laeA-22) was significantly faster than that of the parental strain M. ruber M7, and growth was accompanied by the formation of an abnormal colony phenotype with more abundant aerial hyphae. Interestingly, conidia production of the Mr Delta laeA22 strain was about thrice that of M. ruber M7, but ascospores were not observed in the Mr Delta laeA-22 strain. Additionally, compared to M. ruber M7, Mr Delta laeA-22 exhibited drastically reduced production of multiple secondary metabolites, especially those of the six wellknown Monascus pigments and citrinin. Simultaneously, the selected MrlaeA complementation strain (Mr Delta laeA::laeA-45) nearly recovered the capacity for sporulation and secondary metabolism observed in the parental strain. These results demonstrate that MrLaeA regulates not only secondary metabolism, but also asexual and sexual differentiation in M. ruber, but some of its regulation appears to differ from other fungi. (C) 2015 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.