Villin-like actin-binding proteins are expressed ubiquitously in Arabidopsis
Villin-like actin-binding proteins are expressed ubiquitously in Arabidopsis
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DOI：
10.1104/pp.122.1.35
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发表时间：
2000-01-01
期刊：
影响因子：
7.4
通讯作者：
Chua, NH
中科院分区：
文献类型：
--
作者：
Klahre, U;Friederich, E;Chua, NH
为了阐明植物中绒毛样肌动蛋白结合蛋白的生物学功能，我们克隆了几个与动物绒毛蛋白具有高度同源性的拟南芥蛋白基因。我们发现拟南芥含有至少四个类似绒毛的基因（AtVLN），编码四种不同的VLN亚型。两个AtVLN亚型更密切相关的哺乳动物绒毛蛋白在其一级结构，也抗原相关，而其他两个包含显着的变化，在C-末端头片段结构域。RNA和启动子/β-葡萄糖醛酸酶表达研究表明，AtVLN基因在所有器官中表达，在某些类型的细胞中表达水平升高。这些结果表明，AtVLNs有较少的专门功能比哺乳动物绒毛，这是只发现在刷状缘细胞的微绒毛，免疫印迹实验使用的单克隆抗体对猪绒毛表明，AtVLNs广泛分布在各种植物组织。与全长AtVLN和单个AtVLN头片段结构域融合的绿色荧光蛋白可以在体内与动物和植物肌动蛋白丝结合。
In an attempt to elucidate the biological function of villin-like actin-binding proteins in plants we have cloned several genes encoding Arabidopsis proteins with high homology to animal villin. We found that Arabidopsis contains at least four villin-like genes (AtVLNs) encoding four different VLN isoforms. Two AtVLN isoforms are more closely related to mammalian villin in their primary structure and are also antigenically related, whereas the other two contain significant changes in the C-terminal headpiece domain. RNA and promoter/beta-glucuronidase expression studies demonstrated that AtVLN genes are expressed in all organs, with elevated expression levels in certain types of cells. These results suggest that AtVLNs have less-specialized functions than mammalian villin, which is found only in the microvilli of brush border cells, Immunoblot experiments using a monoclonal antibody against pig villin showed that AtVLNs are widely distributed in a variety of plant tissues. Green fluorescent protein fused to full-length AtVLN and individual AtVLN headpiece domains can bind to both animal and plant actin filaments in vivo.