Time-resolved surface temperature measurement of MALDI matrices under pulsed UV laser irradiation
Time-resolved surface temperature measurement of MALDI matrices under pulsed UV laser irradiation
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DOI：
10.1021/jp037811k
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发表时间：
2004-04-01
影响因子：
2.9
通讯作者：
Zenobi, R
中科院分区：
文献类型：
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作者：
Koubenakis, A;Frankevich, V;Zenobi, R
对受 UV（gimel = 355 nm，tau = 5 ns）激光脉冲照射的各种 MALDI 基质进行表面温度的时间分辨检查。通过检测样品表面发射的黑体辐射来测量温度。观察到最高表面温度出现在激光脉冲的下降沿期间，相对于激光脉冲峰值有 2 ns 的延迟。人们发现它的值取决于基质的种类、样品的厚度和激光能量密度。对于最常用的基质 2,5-二羟基苯甲酸，在本工作中使用的较低能量密度 (10 J/m(2)) 和较高能量密度 (100 J/m(2)) 下，峰值温度分别类似于 850 和 1100 K。
Time-resolved examination of the surface temperature was performed for various MALDI matrices, irradiated by UV (gimel = 355 nm, tau = 5 ns) laser pulses. The temperature was measured by detecting the emitted blackbody radiation from the sample surface. The maximum surface temperature was observed to occur during the falling edge of the laser pulse, with a delay of 2 ns with respect to the laser pulse peak. Its value was found to depend on the kind of the matrix, the thickness of the sample and the laser fluence. For 2,5-dihydroxybenzoic acid, the most popular matrix, the peak temperature was found to be similar to850 and 1100 K at the lower (10 J/m(2)) and the higher (100 J/m(2)) fluences, respectively, used in this work.