Inhibitory Effect of Low-Intensity Pulsed Ultrasound on the Expression of Lipopolysaccharide-Induced Inflammatory Factors in U937 Cells
Inhibitory Effect of Low-Intensity Pulsed Ultrasound on the Expression of Lipopolysaccharide-Induced Inflammatory Factors in U937 Cells
复制标题
低强度脉冲超声对 U937 细胞脂多糖诱导的炎症因子表达的抑制作用。
DOI：
10.1002/jum.14239
复制
发表时间：
2017-12-01
影响因子：
2.3
通讯作者：
Song, Jinlin
中科院分区：
文献类型：
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作者：
Zhang, Xuan;Hu, Bo;Song, Jinlin
低强度脉冲超声(US)可促进牙周组织再生，减少软组织和骨感染性疾病的炎症反应。本实验研究了低强度脉冲超声对脂多糖(LPS)诱导的U937巨噬细胞炎症因子表达的影响。用细胞计数试剂盒和流式细胞仪检测U937细胞的存活率和凋亡率。采用实时定量聚合酶链式反应和酶联免疫吸附试验检测炎症因子的表达。用免疫印迹法检测Toll样受体4、p65、p-IB和IB的表达水平。结果1-g/mLLPS诱导U937细胞肿瘤坏死因子表达开始增加。60 mW/cm(2)的低强度脉冲超声能更有效地降低IL-8的表达。此外，低强度脉冲超声可明显抑制U937细胞的存活和增加其凋亡率(P<0.05)。
ObjectivesLow-intensity pulsed ultrasound (US) has been reported to promote periodontal tissue regeneration and reduce inflammation in soft tissues and in bone infectious diseases. Here we investigated the effect of low-intensity pulsed US on the expression of lipopolysaccharide (LPS)-induced inflammatory factors in U937 macrophage cells.MethodsU937 cells were stimulated with different concentrations of LPS and exposed to different intensities of low-intensity pulsed US. Cell viability and apoptosis of U937 cells were determined by cell-counting kit assays and flow cytometry. A real-time polymerase chain reaction and an enzyme-linked immunosorbent assay were used to test the expression of inflammatory factors. The expression levels of toll-like receptor 4, p65, p-IB, and IB were assessed by western blots.ResultsTumor necrosis factor began to increase in U937 cells on induction with 1-g/mL LPS. Low-intensity pulsed US at the intensity of 60 mW/cm(2) was more effective in reducing interleukin 8 (IL-8) expression. Furthermore, LPS inhibited the viability and increased apoptosis of U937 cells, whereas low-intensity pulsed US significantly reversed these effects (P