Study on the toxicity of phenolic and phenoxy herbicides using the submitochondrial particle assay.
Study on the toxicity of phenolic and phenoxy herbicides using the submitochondrial particle assay.
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使用亚软骨颗粒测定法研究酚类和苯氧基除草剂的毒性。
DOI：
10.1016/j.tiv.2005.05.004
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
P. Ghetti
中科院分区：
文献类型：
--
作者：
E. Argese;C. Bettiol;D. Marchetto;S. De Vettori;A. Zambon;P. Miana;P. Ghetti
一个简单而快速的体外毒理学试验，利用亚线粒体颗粒（SMP），已被用来评估属于苯酚和苯氧基链烷酸化学类的15种除草剂的毒性作用。SMP测定允许定量评价具有不同作用机制的化合物的毒性：解偶联剂、参与反向电子转移和氧化磷酸化的酶复合物的抑制剂以及改变膜结构的化学品。两组除草剂表现出不同程度的毒性。对于苯酚衍生物，EC 50值范围为0.16μM（碘苯腈）至6.7μM（2，4-二硝基苯酚），而对于苯氧基除草剂，EC 50值范围为21μM（2，4，5-三氯苯氧基乙酸，2，4，5-T）至110μM（4-氯-2-甲基苯氧基乙酸，MCPA）。平均而言，酚类化合物的毒性比苯氧基链烷酸的毒性大两个数量级。建立了EC 50值与各种分子描述符之间的定量构效关系（QSAR）。结果表明两类化合物存在不同的作用机制。酚类除草剂的研究结果是一致的质子的解偶联机制，而苯氧基除草剂在膜水平的非特异性的行动模式可以假设。
A simple and rapid in vitro toxicological assay, utilizing submitochondrial particles (SMP), has been used to evaluate the toxic effects of fifteen herbicides belonging to the phenol and phenoxyalkanoic acid chemical classes. The SMP assay allows the quantitative evaluation of the toxicity of compounds with different mechanisms of action: uncouplers, inhibitors of the enzyme complexes involved in reverse electron transfer and in oxidative phosphorylation and chemicals that alter the membrane structure. The two groups of herbicides showed different levels of toxicity. For phenol derivatives, EC50values ranged from 0.16μM (ioxynil) to 6.7μM (2,4-dinitrophenol), whereas for phenoxy herbicides EC50values ranged from 21μM (2,4,5-trichlorophenoxyacetic acid, 2,4,5-T) to 110μM (4-chloro-2-methylphenoxyacetic acid, MCPA). On the average, the toxicity of phenolic compounds is greater than that of phenoxyalkanoic acids by two orders of magnitude. Quantitative structure–activity relationships (QSAR) were developed between EC50values and various molecular descriptors. The results suggest the existence of different mechanisms of action for the two classes of compounds. The findings obtained for phenolic herbicides are consistent with a protonophoric uncoupling mechanism, whereas for phenoxy herbicides a non-specific mode of action at membrane level can be hypothesized.