Preparation of sonoactivated TiO2-DVDMS nanocomposite for enhanced antibacterial activity
Preparation of sonoactivated TiO2-DVDMS nanocomposite for enhanced antibacterial activity
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DOI：
10.1016/j.ultsonch.2020.104968
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发表时间：
2020-05-01
影响因子：
8.4
通讯作者：
Wang, Xiaobing
中科院分区：
文献类型：
--
作者：
Wang, Yihui;Sun, Yue;Wang, Xiaobing
二氧化钛（TiO 2）纳米粒子具有良好的光/声催化性能，但其在溶液中的团聚限制了其在生物医学领域的广泛应用。在本研究中，聚集的二氧化钛纳米粒子的减轻，通过简易的制造在不同的pH值条件下。通过与微量DVDMS敏化剂（DFT）适当偶联，进一步合成了一种新型的TiO 2纳米复合材料。并对其表征、声活性及抗菌效果进行了评价。结果表明，在相同的超声辐照条件下，与单纯的TiO 2表面改性（F-TiO 2）和游离DVDMS相比，DFT的声化学活性有了很大的提高。超声波刺激DFT对细菌有很好的杀灭作用，对S.金黄色。流式细胞仪分析表明，DFT和超声的组合增加了细胞内的ROS和膜扰动。研究结果表明，适当的制造和DVDMS掺入大大提高了TiO 2的声催化过程，和基于超声的DFT生物医学应用值得进一步深入研究。
Titanium dioxide (TiO2) nanoparticle has good photo-/sono-catalytic features, the reunion of this particle in solution-phase generally limits the extensive biomedical application. In the present study, the aggregation of TiO2 nanoparticles was alleviated by facile fabrication under different pH conditions. A novel TiO2 nanocomposite was further synthesized by properly conjugation with trace amount of DVDMS sensitizer (named DFT). The characterization, sonoactivity, as well as the antibacterial efficiency were specially evaluated. The results showed that the sonochemical activity of DFT was greatly improved as compared with the simple surface modification of TiO2 (F-TiO2) and free DVDMS, regarding to the hydroxyl radicals and singlet oxygen yields using the same ultrasound exposure. Moreover, ultrasonic stimulation of DFT exhibited excellent bacterial eradication, with up to 92.41% of killing efficiency in S. aureus. The flow cytometry analysis indicated an increased intracellular ROS and membrane disturbance by combination of DFT and ultrasound. The findings suggest that the proper fabrication and DVDMS incorporation greatly improved the sonocatalytic process of TiO2, and the ultrasound based biomedical applications of DFT deserve future deep investigation.