Developmental and tissue-specific accumulation pattern for the Drosophila melanogaster TART ORF1 protein.
Developmental and tissue-specific accumulation pattern for the Drosophila melanogaster TART ORF1 protein.
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DOI：
10.1016/j.gene.2008.02.002
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发表时间：
2008-05
期刊：
影响因子：
3.5
通讯作者：
P. Maxwell;J. M. Belote;R. Levis
中科院分区：
文献类型：
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作者：
P. Maxwell;J. M. Belote;R. Levis
果蝇非 LTR 逆转录转座子的 TART、HeT-A 和 TAHRE 家族特异性逆转录转座至端粒以维持端粒 DNA。最近的证据表明，RNA 干扰机制可能调节 TART、HeT-A 和 TAHRE 逆转录转座，但端粒逆转录转座子蛋白的发育和组织特异性表达此前尚未被研究过。我们生成了针对 TART ORF1 蛋白 (ORF1p) 的抗血清，并使用这些抗血清检查了果蝇中 TART ORF1p 的表达模式。我们在整个发育过程中检测到 TART ORF1p，并在幼虫晚期和蛹阶段观察到特别高水平的蛋白质。在晚期幼虫中，ORF1p 积聚在脑和成虫盘组织中，而不是在终末分化的幼虫组织中。 ORF1p 在成虫盘中的积累很有趣，因为之前已在成虫盘中检测到 TART 反义 RNA，我们讨论了这些发现对 TART 调节的影响。
The TART, HeT-A, and TAHRE families of Drosophila non-LTR retrotransposons specifically retrotranspose to telomeres to maintain telomeric DNA. Recent evidence indicates that an RNA interference mechanism is likely to regulate TART, HeT-A, and TAHRE retrotransposition, but the developmental and tissue-specific expression of telomeric retrotransposon proteins has not previously been investigated. We have generated antisera against TART ORF1 protein (ORF1p) and used these antisera to examine the pattern of TART ORF1p expression in Drosophila melanogaster. We detected TART ORF1p throughout most of development and observed particularly high levels of protein in late larval and pupal stages. In late-stage larvae, ORF1p accumulates in brain and imaginal discs tissues, rather than in terminally differentiated larval tissues. Accumulation of ORF1p in imaginal discs is intriguing, since TART antisense RNA has previously been detected in imaginal discs, and we discuss the implications of these findings for TART regulation.