Topographic studies of Torpedo acetylcholine receptor subunits as a transmembrane complex.
Topographic studies of Torpedo acetylcholine receptor subunits as a transmembrane complex.
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鱼雷乙酰胆碱受体亚基作为跨膜复合物的形貌研究。
DOI：
10.1073/pnas.77.10.5807
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发表时间：
1980
影响因子：
11.1
通讯作者：
Raftery,MA
中科院分区：
文献类型：
--
作者：
Strader,CD;Raftery,MA
已经研究了来自加州鱼雷的乙酰胆碱受体的四个亚基在电斑细胞突触后膜的细胞外和细胞质面上的暴露。分离出除受体以外不含蛋白质成分的密封膜囊泡，并显示其 95% 的突触表面面向介质。这些制剂中的四个受体亚基对来自外部和内部介质的胰蛋白酶水解的敏感性用于研究亚基在膜的突触和细胞质表面上的暴露。胰蛋白酶产物的十二烷基硫酸钠凝胶电泳显示，所有四个亚基都以相似的程度暴露在细胞外表面。所有四个亚基也暴露在膜的内表面上，但明显的暴露程度随亚基大小而变化，较大的亚基暴露得更多。结果根据受体作为跨膜蛋白复合物的可能的拓扑模型进行了讨论。
The exposure of the four subunits of the acetylcholine receptor from Torpedo californica on both the extracellular and cytoplasmic faces of the postsynaptic membranes of the electroplaque cells has been investigated. Sealed membrane vesicles containing no protein components other than the receptor were isolated and were shown to have 95% of their synaptic surfaces facing the medium. The susceptibility of the four receptor subunits in these preparations to hydrolysis by trypsin both from the external and from the internal medium was used to investigate the exposure of the subunits on the synaptic and cytoplasmic surfaces of the membrane. It was shown by sodium dodecyl sulfate gel electrophoresis of the tryptic products that all four subunits are exposed on the extracellular surface to a similar degree. All four subunits are also exposed on the internal surface of the membrane, but the apparent degree of exposure varies with the subunit size, the larger subunits being more exposed. The results are discussed in terms of a possible topographic model of the receptor as a transmembrane protein complex.