Characterization of clonogenic stromal cells isolated from human endometrium
Characterization of clonogenic stromal cells isolated from human endometrium
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DOI：
10.1530/rep-07-0428
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发表时间：
2008-04-01
期刊：
影响因子：
3.8
通讯作者：
Kyurkchiev, S.
中科院分区：
文献类型：
--
作者：
Dimitrov, R.;Timeva, T.;Kyurkchiev, S.
人类子宫内膜是女性生殖生活中广泛重组和重塑的对象，并且很容易假设这些周期性变化是在具有多能细胞基本特征的细胞的参与下发生的。本研究的目的是寻找人子宫内膜中具有可塑性粘附、克隆形成和分化的细胞的存在。为此，体外培养人子宫内膜间质细胞超过15代。流式细胞仪分析培养的细胞显示，它们对CD 29、CD 73和CD 90呈阳性，这被认为是间充质来源的细胞的标志物。细胞对造血细胞标志物（CD 45、CD 34、CD 14、CD 3. CD 19、CD 16/56和HLA-DR）。此外，显示培养的细胞具有15%的克隆形成效率，并且可以被诱导分化为含有典型的富脂空泡的成脂细胞。这些结果表明，人子宫内膜含有少量具有子宫内膜间质干/祖细胞特征的细胞，其似乎属于间充质干细胞家族。推测这些细胞参与了人子宫内膜每月一次的重构和重塑。
Human endometrium is an object of extensive restructuring and remodeling during the female reproductive life and it is quite tempting to assume that these periodic changes happen with the participation of cells that should have the basic characteristics of multipotent cells. The aim of this study was to search for the presence of cells with plastic adherence, clonogenicity, and differentiation in human endometrium. To this end, human endometrial stromal cells were cultured in vitro for more than 15 passages. Flow cytometry analysis of the cultured cells showed that they were positive for CD29, CD73 and CD90, which are considered to be the markers of cells with mesenchymal origin. The cells were negative for the hematopoietic cell markers (CD45, CD34, CD14, CD3. CD19, CD16/56, and HLA-DR). Further, it was shown that the cultured cells had 15% clonogenic efficiency and could be induced to differentiate into adipogenic cells containing typical lipid-rich vacuoles. These results demonstrate that the human endometrium contains a low number of cells with the characteristics of endometrial stromal stem/progenitor cells, which seem to belong to the family of the mesenchymal stem cells. It can he speculated that these cells are engaged into the monthly restructuring and remodeling of human endometrium.