Inorganic polyphosphate is needed for swimming, swarming, and twitching motilities of Pseudomonas aeruginosa
Inorganic polyphosphate is needed for swimming, swarming, and twitching motilities of Pseudomonas aeruginosa
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DOI：
10.1073/pnas.060030097
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发表时间：
2000-04-25
影响因子：
11.1
通讯作者：
Kornberg, A
中科院分区：
文献类型：
--
作者：
Rashid, MH;Kornberg, A
多聚磷酸酶(PPK)。由PPK基因编码，是许多细菌从三磷酸腺苷(ATP)合成无机多聚磷(PolyP)的主要酶。铜绿假单胞菌PAO1的PPK基因敲除突变体在半固体琼脂平板上的鞭毛游动能力受损。该突变体缺乏IV型药丸介导的抽搐运动和以前在铜绿假单胞菌中未观察到的“成群运动”。在集群培养中，极地单毛细菌已经分化成细长的极地多毛细胞，它们在固体介质表面导航。PPK突变体中的所有运动缺陷都可以由含有PPK基因的质粒来补充。由于细菌的运动性通常对它们在自然环境中的生存和宿主内的系统感染至关重要，因此对PPK运动性的依赖揭示了PolyP在生物被膜形成、共生和毒力等不同过程中的重要作用。
Polyphosphate kinase (PPK). encoded by the ppk gene, is the principal enzyme in many bacteria for the synthesis of inorganic polyphosphate (poly P) from ATP. A knockout mutant in the ppk gene of Pseudomonas aeruginosa PAO1 is impaired in flagellar swimming motility on semisolid agar plates. The mutant is deficient in type IV pill-mediated twitching motility and in a "swarming motility" previously unobserved in P. aeruginosa. In swarming cultures, the polar monotrichous bacteria have differentiated into elongated and polar multitrichous cells that navigate the surface of solid media. All of the motility defects in the ppk mutant could be complemented by a plasmid harboring the ppk gene. Because bacterial motility is often crucial for their survival in a natural environment and for systemic infection inside a host, the dependence for motility on PPK reveals important roles for poly P in diverse processes such as biofilm formation, symbiosis, and virulence.