Retrofit implementation of Zernike phase plate imaging for cryo-TEM
Retrofit implementation of Zernike phase plate imaging for cryo-TEM
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DOI：
10.1016/j.jsb.2011.01.005
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发表时间：
2011-05-01
影响因子：
3
通讯作者：
Danev, Radostin
中科院分区：
文献类型：
--
作者：
Marko, Michael;Leith, ArDean;Danev, Radostin
聚焦相位板成像对于冷冻 TEM 特别有益，因为它可以显着提高图像对比度，而不会造成电子剂量损失，并且简化了图像解释。我们展示了如何使用适当的现有 TEM 来实现相位板冷冻 TEM，并提供薄膜（碳）相位板使用的基本实用介绍。我们指出了相位板操作的潜在缺陷，并讨论了解决方案。我们提供有关评估特定 TEM 的适用性的信息。 (C) 2011 Elsevier Inc. 保留所有权利。
In-focus phase-plate imaging is particularly beneficial for cryo-TEM because it offers a substantial overall increase in image contrast, without an electron dose penalty, and it simplifies image interpretation. We show how phase-plate cryo-TEM can be implemented with an appropriate existing TEM, and provide a basic practical introduction to use of thin-film (carbon) phase plates. We point out potential pitfalls of phase-plate operation, and discuss solutions. We provide information on evaluating a particular TEM for its suitability. (C) 2011 Elsevier Inc. All rights reserved.