Temperature-sensitive carrot variants impaired in somatic embryogenesis.
Temperature-sensitive carrot variants impaired in somatic embryogenesis.
复制标题
温度敏感的胡萝卜变体会损害体细胞胚胎发生。
DOI：
10.1016/0012-1606(82)90211-1
复制
发表时间：
1982
影响因子：
2.7
通讯作者：
Sung,ZR
中科院分区：
文献类型：
--
作者：
Breton,AM;Sung,ZR
许多植物的培养细胞都能再生成植物。在胡萝卜中，植物的再生是通过胚胎发生的。由于大量体细胞胚胎是从单倍体胡萝卜培养物中产生的，因此有可能设计一种选择程序来恢复在早期胚胎发生中受损的温度敏感变异（ts-emb−）。字型线分为三类。第一类在胚性培养基中能发生任何生长之前被阻断；第二类细胞在有组织的生长中受阻：细胞可以以愈伤组织的形式生长，但不能形成胚胎；第三个在胚胎发育的第一阶段，即球形阶段受阻。这些分类有助于描述早期体细胞胚胎发生的发育步骤。大多数变异系的Thets-emb -表型在培养中是稳定的，并通过植株再生得以维持。关于thets-emb -表型的起源提出了假设。除了thets-emb -系外，还发现了其他类型的变异系，即ts-生长系、习惯系和对生长调节剂反应改变的系。
Cultured cells of many plant species are capable of regenerating back to plants. In carrots, plant regeneration takes place via embryogenesis. Because vast numbers of somatic embryos are produced from haploid carrot cultures, it is possible to design a selection procedure for recovering temperature-sensitive variants impaired in early embryogenesis (ts-emb−). Thets-emb−lines fall into three classes. The first class is blocked before any growth in embryogenic medium can take place; the second class is blocked in organized growth: cells can grow as callus, but cannot form embryos; the third is blocked at the first stage of embryo development, the globular stage. These classes are useful in delineating the developmental steps during early somatic embryogenesis. Thets-emb−phenotype of most variant lines is stable in culture and is maintained through plant regeneration. Hypotheses are made concerning the origin of thets-emb−phenotype. In addition to thets-emb−lines, other classes of variants have been recovered, i.e.,ts-growth−, habituated lines, and lines with altered responses to growth regulators.