Affinity purification of specific chromatin segments from chromosomal loci in yeast
Affinity purification of specific chromatin segments from chromosomal loci in yeast
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DOI：
10.1128/mcb.23.24.9275-9282.2003
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发表时间：
2003-12-01
影响因子：
5.3
通讯作者：
Kornberg, RD
中科院分区：
文献类型：
--
作者：
Griesenbeck, J;Boeger, H;Kornberg, RD
从酵母染色体中提取了单拷贝基因和启动子区域，并通过常规方法和亲和方法纯化为染色质。处于转录抑制和激活状态的启动子区域保持其特有的染色质结构。凝胶过滤分析确定了转录激活状态的一致性。激活剂蛋白的相互作用方式与先前活体研究中预期的方式相同。这项工作为直接研究处于不同功能和结构状态的真核细胞染色体的特定基因区域开辟了道路。
Single-copy gene and promoter regions have been excised from yeast chromosomes and have been purified as chromatin by conventional and affinity methods. Promoter regions isolated in transcriptionally repressed and activated states maintain their characteristic chromatin structures. Gel filtration analysis establishes the uniformity of the transcriptionally activated state. Activator proteins interact in the manner anticipated from previous studies in vivo. This work opens the way to the direct study of specific gene regions of eukaryotic chromosomes in diverse functional and structural states.