SINGLE CELL RESPONSES IN FEMALE PIERIS-BRASSICAE (LEPIDOPTERA, PIERIDAE) TO PLANT VOLATILES AND CONSPECIFIC EGG ODORS
SINGLE CELL RESPONSES IN FEMALE PIERIS-BRASSICAE (LEPIDOPTERA, PIERIDAE) TO PLANT VOLATILES AND CONSPECIFIC EGG ODORS
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DOI：
10.1016/0022-1910(80)90117-1
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发表时间：
1980-01-01
影响因子：
2.2
通讯作者：
MAES, FW
中科院分区：
文献类型：
--
作者：
DENOTTER, CJ;BEHAN, M;MAES, FW
利用一种新的“表面接触”记录技术，记录了甘蓝白蝶雌蝶个体嗅觉细胞的动作电位和触角的受体电位。使用的刺激物是植物挥发物和同种鸡蛋气味。大多数细胞被某些刺激激活，而被其他刺激抑制。因此，大多数细胞的“气味光谱”既包括激活物质，也包括抑制物质。此外，特定刺激的“反应轮廓”包括正反应细胞和负反应细胞。气味光谱的聚类分析表明，存在适度分离的嗅觉感受器簇。没有发现专门对鸡蛋或卷心菜叶子的气味进行调节的细胞群。对反应曲线的分析没有显示出明显的刺激聚集。结果表明，不同受体细胞膜上含有不同比例的多种受体部位，而EAG与受体电位幅值和峰频之间无相关性。结论是，只有当涉及化学相关化合物时，EAG才能用于比较相对受体的敏感性。
Action potentials from individual olfactory cells and receptor potentials were recorded from antennae of female cabbage white butterflies,Pieris brassicae, using a new ‘surface-contact’ recording technique. Stimuli used were plant volatiles and conspecific egg odours. Most cells were activated by some stimuli and inhibited by others. Hence, the ‘odour spectra’ of most cells included activating as well as inhibiting substances. In addition, the ‘response profile’ of a given stimulus contained both positively and negatively responding cells.Cluster analysis of the odour spectra revealed the existence of moderately separated clusters of olfactory receptors. No groups of cells specifically tuned to odours of eggs or cabbage leaves were found. Analysis of the response profiles did not show a clear clustering of stimuli. The results suggested that the membranes of different receptor cells contain several types of acceptor sites in various proportions.No correlation between EAG and receptor potential amplitude or spike frequency was found. It was concluded that the EAG may be useful in comparing the relative receptor sensitivities only when chemically related compounds are involved.