Hydrogen and oxygen stable isotope analysis of pollen collected from honey
Hydrogen and oxygen stable isotope analysis of pollen collected from honey
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蜂蜜花粉的氢氧稳定同位素分析
DOI：
10.1080/00173134.2013.841751
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发表时间：
2013
期刊：
影响因子：
0.9
通讯作者：
J. Ehleringer
中科院分区：
文献类型：
--
作者：
L. Chesson;B. Tipple;Brad R. Erkkila;J. Ehleringer
摘要 对复杂基质中花粉的稳定同位素比率进行分析可能有助于样品来源或气候变化应用。我们开发了一种从蜂蜜中分离花粉并制备用于氢氧稳定同位素比分析的花粉的方法。该方法结合多种处理：乙醇稀释分离花粉；己烷洗涤以除去蜡；和丙酮水解以消化除花粉颗粒外层之外的所有花粉颗粒。存在与治疗相关的大同位素效应，但该方法的重复应用之间的效果是一致的。我们使用该方法从美国各地的 45 个蜂蜜样品中分离出花粉。花粉δ2H和δ18O同位素值呈显着正相关（P < 0.05），但δ18O值解释的δ2H值变化不大（r2 = 0.17）。花粉δ2H值与蜂巢预测降水δ2H值显着相关（P < 0.05，r2 = 0.24），但花粉与降水δ18O值之间不存在显着相关性（P = 0.34，r2 = 0.03）。这些结果表明，在调查来源时，花粉的氢和氧稳定同位素分析可能不如液体蜂蜜或蜂蜡分析有用。
Abstract Analyses of stable isotope ratios of pollen present within complex matrices may be useful in sample provenance or climate change applications. We developed a method for separating pollen from honey and preparing pollen for hydrogen and oxygen stable isotope ratio analysis. The method combined several treatments: ethanol dilution to separate pollen; hexane washing to remove wax; and acetolysis to digest all but the external layer of pollen granules. There were large isotopic effects associated with treatments but effects were consistent between replicate applications of the method. We used the method to separate pollen from 45 honey samples from across the United States. Pollen δ2H and δ18O isotope values were significantly positively correlated (P < 0.05), but there was little variation in δ2H values explained by δ18O values (r2 = 0.17). Pollen δ2H values were significantly correlated with δ2H values of precipitation predicted for hive locations (P < 0.05, r2 = 0.24), but there was no significant correlation between pollen and precipitation δ18O values (P = 0.34, r2 = 0.03). These results suggest hydrogen and oxygen stable isotope analysis of pollen may not be as useful as analysis of liquid honey or beeswax in investigating provenance.