Kinesin heavy chain is essential for viability and neuromuscular functions in Drosophila, but mutants show no defects in mitosis.
Kinesin heavy chain is essential for viability and neuromuscular functions in Drosophila, but mutants show no defects in mitosis.
复制标题
驱动蛋白重链对于果蝇的活力和神经肌肉功能至关重要，但突变体没有显示有丝分裂缺陷。
DOI：
10.1016/0092-8674(91)90264-y
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发表时间：
1991
期刊：
影响因子：
64.5
通讯作者：
Raff,EC
中科院分区：
文献类型：
--
作者：
Saxton,WM;Hicks,J;Goldstein,LS;Raff,EC
使用遗传学和免疫定位在果蝇中检查微管运动蛋白驱动蛋白的体内功能。驱动蛋白重链突变 (khc) 会导致异常行为和致命性。突变幼虫在最后的节段表现出活动能力和触觉反应能力的丧失，然后在幼虫或蛹发育过程中出现全身麻痹和死亡。温度敏感等位基因纯合的成年人也表现出活动能力和感觉反应的丧失。数据表明，驱动蛋白功能至关重要，并表明驱动蛋白在神经肌肉系统中具有重要作用，可能作为轴突运输的发动机。更一般细胞功能的可能性仍然存在，但对 khc 突变体中胚胎发生和形态发生的观察表明，尽管驱动蛋白功能受损，但有丝分裂和细胞周期仍可以继续进行。免疫定位表明驱动蛋白可能具有一些一般的细胞功能，但它不是有丝分裂纺锤体的主要成分。
The in vivo function of the microtubule motor protein kinesin was examined in Drosophila using genetics and immunolocalization. Kinesin heavy chain mutations (khc) cause abnormal behavior and lethality. Mutant larvae exhibit loss of mobility and tactile responsiveness in the most posterior segments, followed by general paralysis and death during larval or pupal development. Adults homozygous for a temperaturesensitive allele also exhibit a loss in mobility and sensory responses. The data indicate that kinesln function is essential and suggest that kinesln has an Important role in the neuromuscular system, perhaps as a motor for axonal transport. The possibility of more general cellular functions remains open, but observation of embryogenesis and morphogenesls in khc mutants suggests that mitosis and the cell cycle can proceed in spite of impaired kinesin function. lmmunolocalization suggests that kinesin may have some general cellular functions but that It is not a major component of mitotic spindles.