Simultaneous Concentration Enrichment and Electrophoretic Separation of Weak Acids on a Microchip
Simultaneous Concentration Enrichment and Electrophoretic Separation of Weak Acids on a Microchip
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在微芯片上同时浓缩富集和电泳分离弱酸
DOI：
10.1002/jssc.201100423
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发表时间：
2011
影响因子：
3.1
通讯作者：
Shigeo Suzuki
中科院分区：
文献类型：
--
作者：
Sachio Yamamoto;Yuki Watanabe;Noriaki Nishida;Shigeo Suzuki
据报道，一种使用酸性聚丙烯酰胺凝胶同时浓缩和分离弱酸的方法，该凝胶是在商用聚（甲基丙烯酸甲酯）制造的微芯片的微流体通道中制造的。该方法基于简单的光化学共聚来制造选择性渗透预浓缩器。将聚甲基丙烯酸甲酯制成的微芯片的交叉点充满由丙烯酰胺、N,N'-亚甲基双丙烯酰胺和2-丙烯酰氨基乙醇酸组成的凝胶溶液，并以核黄素作为光催化引发剂。在样品出口通道的交叉点附近，通过氩离子激光束照射进行原位聚合，该激光束也用作荧光检测的光源。电动特性与样品成分和聚丙烯酰胺凝胶上的阴离子基团之间的静电排斥相结合，使得弱酸能够在凝胶塞阴极侧的界面处被截留和浓缩。该方法在 3 分钟内显示浓度因子高达 105。通过对异硫氰酸荧光素标记的氨基酸进行灵敏分析，证明了离子预浓缩器的有效性。
A method for the simultaneous concentration and separation of weak acids using an acidic polyacrylamide gel, fabricated in the microfluidic channel of a commercial poly(methyl methacrylate)‐made microchip, is reported. This approach is based on simple photochemical copolymerization for the fabrication of a permselective preconcentrator. The intersection of the poly(methyl methacrylate)‐made microchip was filled with a gel solution comprising acrylamide,N,N′‐methylene‐bis‐acrylamide, and 2‐acrylamidoglycolic acid, with riboflavin as a photocatalytic initiator.In situpolymerization, near the cross of the sample outlet channel, was performed by irradiation with an argon ion laser beam that is also used as the light source for fluorimetric detection. The electrokinetic properties, combined with electrostatic repulsion between sample components and the anionic groups on the polyacrylamide gel, enable the entrapment and concentration of weak acids at the interface of the cathodic side of the gel plug. This method displays concentration factors of up to 105within 3 min. The effectiveness of the ionic preconcentrator was demonstrated by the sensitive analysis of fluorescein isothiocyanate‐labeled amino acids.