Thermoresponsive Oligo(ethylene glycol)-Based Polymer Brushes on Polymer Monoliths for All-Aqueous Chromatography
Thermoresponsive Oligo(ethylene glycol)-Based Polymer Brushes on Polymer Monoliths for All-Aqueous Chromatography
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DOI：
10.1021/am403510g
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发表时间：
2013-12-11
影响因子：
9.5
通讯作者：
Chen, Yi
中科院分区：
文献类型：
--
作者：
Li, Nan;Qi, Li;Chen, Yi
采用两步原子转移自由基聚合（ATRP）法合成了接枝有温敏性共聚物聚甲基丙烯酸2-（2-甲氧基乙氧基）乙酯（MEO（2）MA）-共聚低聚乙二醇甲基丙烯酸酯（OEGMA）的多孔聚合物整体材料。通过元素分析、扫描电子显微镜和压汞法对接枝共聚物进行了表征。它们进一步用作全水高效液相色谱（HPLC）的温敏固定相。三种甾体化合物的色谱图表明，接枝共聚物的链长，这是由不同的接枝时间调节，可以影响分离提供不同量的疏水相互作用位点与分析物。此外，类固醇在固定相上的洗脱曲线也可以通过共聚单体组合物来调节。结果表明，多孔聚合物整料能够在等度条件下在纯水移动的相中分离测试混合物。此外，所提出的方法提供了一个简单的和有前途的工具，在设计和构建响应表面的色谱应用。
Porous polymer monoliths onto which were grafted a thermoresponsive copolymer, poly(2-(2-methoxyethoxy)ethyl methacrylate (MEO(2)MA)-co-oligo(ethylene glycol) methacrylate (OEGMA)), were synthesized by the two-step atom transfer radical polymerization (ATRP) method. The copolymer-grafted monoliths were characterized by elemental analysis, scanning electron microscopy, and mercury intrusion porosimetry. They were further used as the thermoresponsive stationary phase for all-aqueous high-performance liquid chromatography (HPLC). The chromatograms of three steroids demonstrated that the chain length of the grafted copolymer, which was regulated by varying the grafting time, could affect the separation by providing different amounts of hydrophobic interaction sites with analytes. Additionally, the elution profiles of steroids on the stationary phase could also be tuned by the comonomer composition. The results showed that the porous polymer monoliths enabled separation of the test mixture in pure aqueous mobile phase under isocratic conditions. Furthermore, the proposed method provides a simple and promising tool in the design and construction of responsive surfaces for chromatography applications.