Detecting single stranded DNA with a solid state nanopore
Detecting single stranded DNA with a solid state nanopore
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DOI：
10.1021/nl051199m
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发表时间：
2005-10-01
期刊：
影响因子：
10.8
通讯作者：
Li, JL
中科院分区：
文献类型：
--
作者：
Fologea, D;Gershow, M;Li, JL
通过在pH值大于约11.6的条件下操作纳米孔探测器，电压偏置固态纳米孔被用于检测和表征固定微米长度的单个单链DNA分子。观察到的分子事件持续时间和阻断电流的分布表明，很大一部分事件遵循恒定事件电荷缺失（ecd）规则，这表明它们对应于以折叠和展开构象分布穿过纳米孔的分子。令人惊讶的是，有很大一部分是展开的。这一结果是开发用于单分子测序应用的固态纳米孔的一个重要里程碑。
Voltage biased solid-state nanopores are used to detect and characterize individual single stranded DNA molecules of fixed micrometer length by operating a nanopore detector at pH values greater than similar to 11.6. The distribution of observed molecular event durations and blockade currents shows that a significant fraction of the events obey a rule of constant event charge deficit (ecd) indicating that they correspond to molecules translocating through the nanopore in a distribution of folded and unfolded configurations. A surprisingly large component is unfolded. The result is an important milestone in developing solid-state nanopores for single molecule sequencing applications.