Antimicrobial susceptibilities of Escherichia coli strains from a turkey operation
Antimicrobial susceptibilities of Escherichia coli strains from a turkey operation
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DOI：
10.2460/javma.2002.221.411
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发表时间：
2002-08-01
期刊：
影响因子：
--
通讯作者：
Sriranganathan, N
中科院分区：
文献类型：
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作者：
Altekruse, SF;Elvinger, F;Sriranganathan, N
目的：测定火鸡操作中分离的17种抗菌素对大肠埃希菌的最低抑菌浓度(MIC)，并评估小样本是否能准确估计几何平均MIC。设计前瞻性研究：样本群体--来自禽类的105个临床分离株和来自20个鸡群(鸡和育肥母鸡)的1104个粪便分离株。我们从每个肥育鸡群中随机抽取了5、10、15、20、25、30、35、40和45个分离株，并从5、7、10和20个群中随机抽取了100到105个分离株。结果：12只鸡中有6只和20只育肥鸡中有18只接受了抗菌药物治疗，通常是因为与大肠杆菌病相一致的呼吸道疾病。所有的鸟都在孵化场接受了庆大霉素。更多的粪便分离株比临床分离株对氨苄西林耐药；然而，更多的临床分离株对环丙沙星、庆大霉素和磺胺甲恶唑耐药。当每个鸡群获得大于或等于15个粪便分离物时，以及当从7个鸡群获得105个分离物时，对鸡群几何平均MIC的精确估计。结论和临床相关性-抗菌药物的使用是常见的，可能是导致分离物耐药模式的原因。通过适度分配实验室资源，生产商可以监测临床和粪便大肠杆菌的抗菌素敏感性，以管理抗菌剂使用和耐药性的风险。
Objective-To measure minimum inhibitory concentrations (MIC) of 17 antimicrobials for Escherichia coli isolates from a turkey operation and assess whether small samples provide precise estimates of geometric mean MIC.Design-Prospective study.Sample Population-105 clinical isolates from birds and 1,104 fecal isolates from 20 flocks (poults and finisher hens).Procedure-A Mueller-Hinton broth dilution panel was used to measure MIC, and MIC of fecal and clinical isolates were compared. We drew random samples of 5, 10, 15, 20, 25, 30, 35, 40, and 45 isolates from each finisher flock and between 100 and 105 isolates from 5, 7, 10, and 20 flocks. Antimicrobial usage was determined for enrolled flocks.Results-Six of 12 poult and 18 of 20 finisher flocks had been treated with antimicrobials, often for respiratory illnesses consistent with colibacillosis. All birds received gentamicin at the hatchery. More fecal than clinical isolates were resistant to ampicillin; however, more clinical isolates were resistant to ciprofloxacin, gentamicin, and sulfamethoxazole. Precise estimates of geometric mean MIC for flocks were obtained when greater than or equal to 15 fecal isolates were obtained per flock and, for the operation, when 105 isolates were obtained from 7 flocks.Conclusion and Clinical Relevance-Antimicrobial usage was common and may have contributed to the resistance patterns of isolates. With a modest allocation of laboratory resources, producers can monitor antimicrobial susceptibilities of clinical and fecal E coli to manage risks of antimicrobial usage and resistance.